An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

Ben T Miles; Alex B Greenwood; Brian R Patton; Henkjan Gersen

doi:10.1021/acsphotonics.5b00732

An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

Ben T Miles, Alex B Greenwood, Brian R Patton, Henkjan Gersen

Research output: Contribution to journal › Article (Academic Journal) › peer-review

6 Citations (Scopus)

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Abstract

Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres.

Original language	English
Pages (from-to)	343–348
Number of pages	6
Journal	ACS Photonics
Volume	3
Issue number	3
Early online date	4 Feb 2016
DOIs	https://doi.org/10.1021/acsphotonics.5b00732
Publication status	Published - 8 Feb 2016

Keywords

Cross-Polarisation Imaging
NANODIAMOND
nv centers
single particle detection
single molecule detection
colocalisation

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10.1021/acsphotonics.5b00732

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This is the author accepted manuscript (AAM). The final published version (version of record) is available online via ACS at http://pubs.acs.org/doi/abs/10.1021/acsphotonics.5b00732. Please refer to any applicable terms of use of the publisher.
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6 Citations
4 Article (Academic Journal)

Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy
Miles, B. T., Greenwood, A. B., Benito-Alifonso, D., Tanner, H., Galan, M. C., Verkade, P. & Gersen, H., 20 Mar 2017, In: Scientific Reports. 7, 7 p., 44666.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

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11 Citations (Scopus)

312 Downloads (Pure)
On the complex point spread function in interferometric cross-polarisation microscopy
Miles, B. T., Hong, X. & Gersen, H., 20 Jan 2015, In: Optics Express. 23, 2, p. 1232-1239 8 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

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12 Citations (Scopus)

468 Downloads (Pure)
On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.
Miles, B., Robinson, E., van Dijk, E. MPH., Lindsay, I., van Hulst, N. F. & Gersen, H., 17 Nov 2015, In: ACS Photonics. 2, 12, p. 1705-1711 7 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

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9 Citations (Scopus)

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1 Finished

ULTRASENSITIVE DETECTION OF NANOMETER SIZED METAL PARTICLES IN LIVING CELLS
Gersen, H.
1/05/08 → 1/05/11
Project: Research

Dr Henkjan Gersen
- H.Gersenbristol.acuk
- School of Physics - Honorary Industrial Professor
- Fundamental Bioscience
- The Bristol Centre for Nanoscience and Quantum Information
Person: Member, Honorary and Visiting Academic

Cite this

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title = "An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds",

abstract = "Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres. ",

keywords = "Cross-Polarisation Imaging, NANODIAMOND, nv centers, single particle detection, single molecule detection, colocalisation",

author = "Miles, {Ben T} and Greenwood, {Alex B} and Patton, {Brian R} and Henkjan Gersen",

year = "2016",

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language = "English",

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AU - Miles, Ben T

AU - Greenwood, Alex B

AU - Patton, Brian R

AU - Gersen, Henkjan

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Y1 - 2016/2/8

N2 - Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres.

AB - Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres.

KW - Cross-Polarisation Imaging

KW - NANODIAMOND

KW - nv centers

KW - single particle detection

KW - single molecule detection

KW - colocalisation

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DO - 10.1021/acsphotonics.5b00732

M3 - Article (Academic Journal)

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JO - ACS Photonics

JF - ACS Photonics

SN - 2330-4022

IS - 3

ER -

An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

Abstract

Keywords

Access to Document

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Research output

Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy

On the complex point spread function in interferometric cross-polarisation microscopy

On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.

Projects

ULTRASENSITIVE DETECTION OF NANOMETER SIZED METAL PARTICLES IN LIVING CELLS

Profiles

Dr Henkjan Gersen

Cite this