An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

Ben T Miles; Alex B Greenwood; Brian R Patton; Henkjan Gersen

doi:10.1021/acsphotonics.5b00732

An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

Ben T Miles
, Alex B Greenwood
, Brian R Patton
, Henkjan Gersen

Research output: Contribution to journal › Article (Academic Journal) › peer-review

8 Citations (Scopus)

573 Downloads (Pure)

Abstract

Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres.

Original language	English
Pages (from-to)	343–348
Number of pages	6
Journal	ACS Photonics
Volume	3
Issue number	3
Early online date	4 Feb 2016
DOIs	https://doi.org/10.1021/acsphotonics.5b00732
Publication status	Published - 8 Feb 2016

Keywords

Cross-Polarisation Imaging
NANODIAMOND
nv centers
single particle detection
single molecule detection
colocalisation

Access to Document

10.1021/acsphotonics.5b00732

Full-text PDF (accepted author manuscript)
This is the author accepted manuscript (AAM). The final published version (version of record) is available online via ACS at http://pubs.acs.org/doi/abs/10.1021/acsphotonics.5b00732. Please refer to any applicable terms of use of the publisher.
Accepted author manuscript, 1.79 MBLicence: CC BY-NC

Handle.net

Persistent link

8 Citations
4 Article (Academic Journal)

Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy
Miles, B. T., Greenwood, A. B., Benito-Alifonso, D., Tanner, H., Galan, M. C., Verkade, P. & Gersen, H., 20 Mar 2017, In: Scientific Reports. 7, 7 p., 44666.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
File
14 Citations (Scopus)

409 Downloads (Pure)
On the complex point spread function in interferometric cross-polarisation microscopy
Miles, B. T., Hong, X. & Gersen, H., 20 Jan 2015, In: Optics Express. 23, 2, p. 1232-1239 8 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
File
12 Citations (Scopus)

563 Downloads (Pure)
On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.
Miles, B., Robinson, E., van Dijk, E. M., Lindsay, I., van Hulst, N. F. & Gersen, H., 17 Nov 2015, In: ACS Photonics. 2, 12, p. 1705-1711 7 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
File
11 Citations (Scopus)

473 Downloads (Pure)

1 Finished

ULTRASENSITIVE DETECTION OF NANOMETER SIZED METAL PARTICLES IN LIVING CELLS
Gersen, H. (Principal Investigator)
1/05/08 → 1/05/11
Project: Research

Dr Henkjan Gersen
- H.Gersenbristol.acuk
- School of Physics - Honorary Industrial Professor
Person: Honorary and Visiting Academic

Cite this

@article{36ebd2b326464b6f819c1aaba478bf4f,

title = "An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds",

abstract = "Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (\textasciitilde{}10nm) with the fluorescent signature from NV- centres. ",

keywords = "Cross-Polarisation Imaging, NANODIAMOND, nv centers, single particle detection, single molecule detection, colocalisation",

author = "Miles, \{Ben T\} and Greenwood, \{Alex B\} and Patton, \{Brian R\} and Henkjan Gersen",

year = "2016",

month = feb,

day = "8",

doi = "10.1021/acsphotonics.5b00732",

language = "English",

volume = "3",

pages = "343–348",

journal = "ACS Photonics",

issn = "2330-4022",

publisher = "American Chemical Society",

number = "3",

}

TY - JOUR

T1 - An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

AU - Miles, Ben T

AU - Greenwood, Alex B

AU - Patton, Brian R

AU - Gersen, Henkjan

PY - 2016/2/8

Y1 - 2016/2/8

N2 - Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres.

AB - Nitrogen-vacancy (NV-) defects embedded in nanodiamond have attracted attention for their useful photonic and spin properties, and their exceptional photo-stability. Efficiently detecting nanodiamonds that possess colour centres and discriminating from any background fluorescent contamination is essential for nanodiamond-based technologies, and so necessitates the detection of both the nanoparticle and the fluorescent signature. However, optically detecting small nanodiamonds (<40 nnm) proves difficult due to the low absorption and scattering cross section of nanodiamonds. Here we demonstrate an all optical method capable of simultaneous colocalising scattered signal from individual nanodiamonds (~10nm) with the fluorescent signature from NV- centres.

KW - Cross-Polarisation Imaging

KW - NANODIAMOND

KW - nv centers

KW - single particle detection

KW - single molecule detection

KW - colocalisation

U2 - 10.1021/acsphotonics.5b00732

DO - 10.1021/acsphotonics.5b00732

M3 - Article (Academic Journal)

SN - 2330-4022

VL - 3

SP - 343

EP - 348

JO - ACS Photonics

JF - ACS Photonics

IS - 3

ER -

An All-Optical Method For Characterising Individual Fluorescent Nanodiamonds

Abstract

Keywords

Access to Document

Handle.net

Fingerprint

Research output

Direct evidence of lack of colocalisation of fluorescently labelled gold labels used in correlative light electron microscopy

On the complex point spread function in interferometric cross-polarisation microscopy

On the sensitivity of Interferometric Cross-Polarisation Microscopy for nanoparticle detection in the near-infrared.

Projects

ULTRASENSITIVE DETECTION OF NANOMETER SIZED METAL PARTICLES IN LIVING CELLS

Profiles

Dr Henkjan Gersen

Cite this