An Alternative Method to Niskin Sampling for Molecular Analysis of the Marine Environment

Jonathan Teague, Tom Scott, Michael Allen, Sanjay Sharma, George Graham

Research output: Contribution to journalSpecial issue (Academic Journal)peer-review

1 Citation (Scopus)
252 Downloads (Pure)

Abstract

The development of low-cost, open-source Remotely Operated Vehicle (ROV) systems has provided almost unrestricted access for researchers looking to monitor the marine environment in ever greater resolution. Sampling microbial communities from the marine environment, however, still usually relies on Niskin-bottle sampling (ROV or Conductivity-Temperature-Depth sampler (CTD) based), a method which introduces an inaccuracy and variability that is incompatible with metatranscriptomic analysis, for example. Here, we describe a versatile, easily-replicated platform which achieves in situ mRNA preservation, via the addition of RNAlater to filtered microbial cells, to enhance ROV or CTD functionality.
Original languageEnglish
Article numberjmse5020022
Number of pages4
JournalJournal of Marine Science and Engineering
Volume5
Issue number2
DOIs
Publication statusPublished - 2 Jun 2017

Keywords

  • remotely operated vehicle
  • Niskin
  • metatranscriptomics

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