An E2-F12 complex is required for intracellular enveloped virus morphogenesis during vaccinia infection

Mark P Dodding, Timothy P Newsome, Lucy M Collinson, Ceri Edwards, Michael Way

Research output: Contribution to journalArticle (Academic Journal)

30 Citations (Scopus)

Abstract

The vaccinia virus protein, F12, has been suggested to play an important role in microtubule-based transport of intracellular enveloped virus (IEV). We found that GFP-F12 is recruited to IEV moving on microtubules but is released from virus particles when they switch to actin-based motility. In the absence of F12, although the majority of IEV remain close to their peri-nuclear site of assembly, a small number of IEV still move with linear trajectories at speeds of 0.85 μm s(-1) , consistent with microtubule transport. Using a recombinant virus expressing GST-F12, we found that the viral protein E2 interacts directly with F12. In infected cells, GFP-E2 is observed on moving IEV as well as in the Golgi region, but is not associated with actin tails. In the absence of E2L, IEV accumulate in the peri-nuclear region and F12 is not recruited. Conversely, GFP-E2 is not observed on IEV in the absence of F12. Ultra-structural analysis of ΔE2L- and ΔF12L-infected cells reveals that loss of either protein results in defects in membrane wrapping during IEV formation. We suggest that E2 and F12 function as a complex that is necessary for IEV morphogenesis prior to their microtubule-based transport towards the plasma membrane.

Original languageEnglish
Pages (from-to)808-24
Number of pages17
JournalCellular Microbiology
Volume11
Issue number5
DOIs
Publication statusPublished - May 2009

Keywords

  • Cell Line
  • Green Fluorescent Proteins
  • Humans
  • Microtubules
  • Protein Transport
  • Vaccinia virus
  • Viral Proteins
  • Virion
  • Journal Article

Fingerprint Dive into the research topics of 'An E2-F12 complex is required for intracellular enveloped virus morphogenesis during vaccinia infection'. Together they form a unique fingerprint.

  • Cite this