An electrophysiological characterisation of long-term potentiation in cultured dissociated hippocampal neurones

SM Fitzjohn, LA Pickard, JK Duckworth, E Molnar, JM Henley, GL Collingridge, JR Noël

Research output: Contribution to journalArticle (Academic Journal)peer-review

30 Citations (Scopus)

Abstract

Long-term potentiation (LTP) of synaptic transmission is under intense investigation. It is believed that the mechanisms involved in its induction and expression are critically involved in synaptic processes that are important for learning and memory and other physiological functions. A reliable means of inducing LTP in dissociated cultured neurones would facilitate investigations into the molecular basis of LTP but has been hard to achieve. Here we report a mechanism for inducing LTP in postnatal dissociated hippocampal neurones using transient depolarisation. This form of LTP is prevented by NMDA receptor antagonists and by chelating Ca2+ in the postsynaptic neurone. It is manifest primarily as an increase in the frequency of mEPSCs.
Translated title of the contributionAn electrophysiological characterisation of long-term potentiation in cultured dissociated hippocampal neurones
Original languageEnglish
Pages (from-to)693 - 699
Number of pages7
JournalNeuropharmacology
Volume41 (6)
DOIs
Publication statusPublished - Nov 2001

Bibliographical note

Publisher: Elsevier

Fingerprint Dive into the research topics of 'An electrophysiological characterisation of long-term potentiation in cultured dissociated hippocampal neurones'. Together they form a unique fingerprint.

Cite this