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An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

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An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells. / Trakarnsanga, Kongtana; Griffiths, Rebecca E; Wilson, Marieangela C; Blair, Allison; Satchwell, Timothy J; Meinders, Marjolein; Cogan, Nicola; Kupzig, Sabine; Kurita, Ryo; Nakamura, Yukio; Toye, Ashley M; Anstee, David J; Frayne, Jan.

In: Nature Communications, Vol. 8, 14750, 14.03.2017.

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Trakarnsanga, Kongtana ; Griffiths, Rebecca E ; Wilson, Marieangela C ; Blair, Allison ; Satchwell, Timothy J ; Meinders, Marjolein ; Cogan, Nicola ; Kupzig, Sabine ; Kurita, Ryo ; Nakamura, Yukio ; Toye, Ashley M ; Anstee, David J ; Frayne, Jan. / An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells. In: Nature Communications. 2017 ; Vol. 8.

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@article{64339c4e5b8e4bbf9508eeff7d3197a6,
title = "An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells",
abstract = "With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.",
keywords = "synthetic biology",
author = "Kongtana Trakarnsanga and Griffiths, {Rebecca E} and Wilson, {Marieangela C} and Allison Blair and Satchwell, {Timothy J} and Marjolein Meinders and Nicola Cogan and Sabine Kupzig and Ryo Kurita and Yukio Nakamura and Toye, {Ashley M} and Anstee, {David J} and Jan Frayne",
year = "2017",
month = "3",
day = "14",
doi = "10.1038/ncomms14750",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer Nature",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells

AU - Trakarnsanga, Kongtana

AU - Griffiths, Rebecca E

AU - Wilson, Marieangela C

AU - Blair, Allison

AU - Satchwell, Timothy J

AU - Meinders, Marjolein

AU - Cogan, Nicola

AU - Kupzig, Sabine

AU - Kurita, Ryo

AU - Nakamura, Yukio

AU - Toye, Ashley M

AU - Anstee, David J

AU - Frayne, Jan

PY - 2017/3/14

Y1 - 2017/3/14

N2 - With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.

AB - With increasing worldwide demand for safe blood, there is much interest in generating red blood cells in vitro as an alternative clinical product. However, available methods for in vitro generation of red cells from adult and cord blood progenitors do not yet provide a sustainable supply, and current systems using pluripotent stem cells as progenitors do not generate viable red cells. We have taken an alternative approach, immortalizing early adult erythroblasts generating a stable line, which provides a continuous supply of red cells. The immortalized cells differentiate efficiently into mature, functional reticulocytes that can be isolated by filtration. Extensive characterization has not revealed any differences between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the molecular level, and importantly no aberrant protein expression. We demonstrate a feasible approach to the manufacture of red cells for clinical use from in vitro culture.

KW - synthetic biology

U2 - 10.1038/ncomms14750

DO - 10.1038/ncomms14750

M3 - Article

C2 - 28290447

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 14750

ER -