The purpose of this study was to characterize the spatial distribution, number and size of CD34+ cells in fetal bone marrow. Thin sections of normal fetal bone marrow from lumbar vertebrae were stained using CD34 antibody QBend/10. Sections were used under light microscopy with various eyepiece graticules to make measurements of CD34+ cells in situ. Results showed that at mid- and late gestation, approximately 2% and 0Â·5% of fetal bone marrow cells were CD34+ respectively. The mean distance of CD34+ cells from the nearest trabecular bone surface was 61 Â± 4 and 46 Â± 4 Î¼m, respectively, for mid- and late gestation. The mean distance to the nearest neighbour was 46 Â± 5 and 105 Â± 15 Î¼m, and the mean distance to the nearest blood vessel was 13 Â± 1 and 17 Â± 2 Î¼m respectively. The concentration of CD34+ cells in the peripheral region was 6Â·5 times greater than that at the centre of the sections. Overall, the percentage number of CD34+ cells decreased with gestational age. The cellular and nuclear diameters of CD34+ cells remained unchanged throughout mid- and late gestation at 5Â·4 Â± 0Â·1 and 3Â·8 Â± 0Â·1 Î¼m respectively. This information will be used to calculate the natural background alpha-radiation dose to haemopoietic stem cells.
|Pages (from-to)||201 - 210|
|Number of pages||10|
|Journal||British Journal of Haematology|
|Publication status||Published - 2001|