An optimised protocol for the production of autologous serum eyedrops

Lei Liu, D Hartwig, S Harloff, P Herminghaus, T Wedel, G Geerling

Research output: Contribution to journalArticle (Academic Journal)

103 Citations (Scopus)

Abstract

Serum eyedrops have been successfully used in the treatment of severe dry eye, persistent epithelial defects and other severe ocular surface disorders. A number of clinical studies showed a variable efficacy of this approach, but the parameters for the production of this blood product varied significantly. In order to establish an optimised protocol for the production of serum eyedrops, we examined the effect of various clotting times, centrifugation forces, types of diluent and dilutions on the concentration of growth factors, fibronectin, and vitamins in serum and tested the epitheliotrophic capacity of these serum modifications in a cell culture model of human SV-40-immortalised corneal epithelial cells (HCE-T).
Original languageEnglish
Pages (from-to)706-14
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume243
Issue number7
DOIs
Publication statusPublished - Jul 2005

Keywords

  • Cell Movement
  • Growth Substances
  • Humans
  • Aged
  • Cell Differentiation
  • Cell Proliferation
  • Epithelium, Corneal
  • Chromatography, High Pressure Liquid
  • Centrifugation
  • Vitamin E
  • Serum
  • Vitamin A
  • Adult
  • Ophthalmic Solutions
  • Enzyme-Linked Immunosorbent Assay
  • Middle Aged
  • Male
  • Clinical Protocols
  • Female
  • Thrombin Time

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