Analysis and control of genetic toggle switches subject to periodic multi-input stimulation

Davide Fiore, Agostino Guarino, Mario Di Bernardo*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

9 Citations (Scopus)
101 Downloads (Pure)

Abstract

In this letter, we analyze a genetic toggle switch recently studied in the literature where the expression of two repressor proteins can be tuned by controlling two different inputs, namely the concentration of two inducer molecules in the growth medium of the cells. Specifically, we investigate the dynamics of this system when subject to pulse-width modulated inputs. We provide an analytical model that captures qualitatively the experimental observations reported in the literature and approximates its asymptotic behavior. We also discuss the effect that the system parameters have on the prediction accuracy of the model. Moreover, we propose a possible external control strategy to regulate the mean value of the fluorescence of the reporter proteins when the cells are subject to such periodic forcing.

Original languageEnglish
Article number8456594
Pages (from-to)278-283
Number of pages6
JournalIEEE Control Systems Letters
Volume3
Issue number2
Early online date6 Nov 2018
DOIs
Publication statusPublished - 1 Apr 2019

Keywords

  • genetic regulatory systems
  • modeling
  • Systems biology

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