TY - JOUR
T1 - Analysis and control of genetic toggle switches subject to periodic multi-input stimulation
AU - Fiore, Davide
AU - Guarino, Agostino
AU - Di Bernardo, Mario
PY - 2019/4/1
Y1 - 2019/4/1
N2 - In this letter, we analyze a genetic toggle switch recently studied in the literature where the expression of two repressor proteins can be tuned by controlling two different inputs, namely the concentration of two inducer molecules in the growth medium of the cells. Specifically, we investigate the dynamics of this system when subject to pulse-width modulated inputs. We provide an analytical model that captures qualitatively the experimental observations reported in the literature and approximates its asymptotic behavior. We also discuss the effect that the system parameters have on the prediction accuracy of the model. Moreover, we propose a possible external control strategy to regulate the mean value of the fluorescence of the reporter proteins when the cells are subject to such periodic forcing.
AB - In this letter, we analyze a genetic toggle switch recently studied in the literature where the expression of two repressor proteins can be tuned by controlling two different inputs, namely the concentration of two inducer molecules in the growth medium of the cells. Specifically, we investigate the dynamics of this system when subject to pulse-width modulated inputs. We provide an analytical model that captures qualitatively the experimental observations reported in the literature and approximates its asymptotic behavior. We also discuss the effect that the system parameters have on the prediction accuracy of the model. Moreover, we propose a possible external control strategy to regulate the mean value of the fluorescence of the reporter proteins when the cells are subject to such periodic forcing.
KW - genetic regulatory systems
KW - modeling
KW - Systems biology
UR - http://www.scopus.com/inward/record.url?scp=85056400588&partnerID=8YFLogxK
UR - https://arxiv.org/abs/1806.02171
U2 - 10.1109/LCSYS.2018.2868925
DO - 10.1109/LCSYS.2018.2868925
M3 - Article (Academic Journal)
AN - SCOPUS:85056400588
VL - 3
SP - 278
EP - 283
JO - IEEE Control Systems Letters
JF - IEEE Control Systems Letters
SN - 2475-1456
IS - 2
M1 - 8456594
ER -