Analysis of gene expression in canine idiopathic pulmonary fibrosis

E. Krafft*, H. P. Laurila, I. R. Peters, F. Bureau, D. Peeters, M. J. Day, M. M. Rajamaki, C. Clercx

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

8 Citations (Scopus)

Abstract

Idiopathic pulmonary fibrosis (IPF) in dogs is a rare disease of unknown aetiology, seen in terrier breeds, particularly the West Highland white terrier (WHWT). The aim of this study was to determine pulmonary gene expression in canine IPF in order to gain insights into the pathogenesis of the disease and to identify possible biomarkers. Microarray analyses were conducted to determine gene expression profiles in the lungs of dogs with IPF and control dogs of various breeds. More than 700 genes were identified as having greater than two-fold difference in expression between the two groups. The significant biological functions associated with these genes were related to cellular growth and proliferation, developmental processes, cellular movement, cell to cell signalling and interaction, and antigen presentation. Altered levels of expression were confirmed by quantitative reverse transcriptase PCR for genes encoding chemokine (C-C) ligand (CCL) 2 (+4.9 times), CCL7 (+6.8 times), interleukin 8 (+4.32 times), chemokine (C-X-C) ligand 14 (+3.4 times), fibroblast activation protein (+4.7 times) and the palate, lung and nasal associated protein (PLUNC, 25 times). Serum CCL2 concentrations were significantly higher in WHWTs with IPF (mean 628.1 pg/mL, interquartile range 460.3-652.7 pg/mL) than unaffected WHWTs (mean 344.0 pg/mL, interquartile range 254.5-415.5 pg/mL; P = 0.001). The results support CCL2 as a candidate biomarker for IPF in dogs. (C) 2013 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)479-486
Number of pages8
JournalVeterinary Journal
Volume198
Issue number2
DOIs
Publication statusPublished - Nov 2013

Keywords

  • Canine
  • Idiopathic pulmonary fibrosis
  • Microarray analysis
  • Gene expression
  • Chemokine ligand 2
  • HIGHLAND WHITE TERRIERS
  • TIME RT-PCR
  • MONOCYTE CHEMOATTRACTANT PROTEIN-1
  • FIBROBLAST ACTIVATION PROTEIN
  • USUAL INTERSTITIAL PNEUMONIA
  • PERIPHERAL-BLOOD BIOMARKERS
  • RNA ENCODING CYTOKINES
  • FASL-INDUCED APOPTOSIS
  • MESSENGER-RNA
  • EPITHELIAL-CELLS

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