Analysis of risk factors and prevalence of haemoplasma infection in dogs

L. C. Aquino*, J. Kamani, A. M. Haruna, G. R. Paludo, C. A. Hicks, C. R. Helps, S. Tasker

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

16 Citations (Scopus)
266 Downloads (Pure)

Abstract

Mycoplasma haemocanis (Mhc) and 'Candidatus Mycoplasma haematoparvum' (CMhp) are canine haemoplasma species that can induce anaemia in immunocompromised and/or splenectomised dogs. This study aimed to determine the prevalence and phylogeny of canine haemoplasma species in dogs from Nigeria and describe any risk factors for infection. Canine haemoplasma species-specific and generic haemoplasma qPCR assays were used. The species-specific qPCR assays found Mhc infection in 18 of 245 dogs (7.3%), and CMhp infection in only one dog (0.4%). The generic haemoplasma qPCR assays were positive in 44 of 245 (17.9%) dogs. Twenty-five dogs had discordant qPCR results in that they were generic haemoplasma qPCR positive but species-specific qPCR negative. Further evaluation of these dogs by 16S rDNA sequencing gave limited results but 5 were confirmed to be infected with non-haemoplasma species: 2 Anaplasma phagocytophilum, 1 Anaplasma ovis, 1 Serratia marcescens and 1 Aerococcus spp. The 16S rRNA gene sequences from Mhc species showed > 99.8% identity with each other and > 99.6% identity with GenBank sequences, and resided in a single clade with other global Mhc and Mycoplasma haemofelis sequences, indicating low 16S rRNA genetic variability amongst this canine haemoplasma species.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalVeterinary Parasitology
Volume221
DOIs
Publication statusPublished - 16 May 2016

Keywords

  • Dogs
  • Haemoplasmas
  • Nigeria
  • Prevalence
  • Risk factors

Fingerprint

Dive into the research topics of 'Analysis of risk factors and prevalence of haemoplasma infection in dogs'. Together they form a unique fingerprint.

Cite this