Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay

Audrey Colomba; Anne J Ridley

doi:10.1007/978-1-62703-791-4_21

Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay

Audrey Colomba, Anne J Ridley

Research output: Contribution to journal › Article (Academic Journal) › peer-review

6 Citations (Scopus)

Abstract

Rho GTPases are master regulators of cytoskeleton dynamics and therefore regulate cell motility. Rho GTPases, as well as their regulators and effectors, are often deregulated in cancers and thus contribute to tumor progression to metastasis. Cancer progression involves multiple steps, including invasion of the surrounding tissues. Several methods to investigate the invasion of tumors cells in 3D matrices in vitro have been developed. In this chapter we describe a 3D-based morphology assay that can be used for medium-throughput microscopy-based screening to identify regulators of cancer cell invasion. We use this method coupled to RNAi to investigate how Rho GTPases affect prostate cancer cell morphology in 3D Matrigel.

Original language	English
Pages (from-to)	327-37
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1120
DOIs	https://doi.org/10.1007/978-1-62703-791-4_21
Publication status	Published - 2014

Keywords

Cell Line, Tumor
Cell Movement
Cell Survival
Collagen
Drug Combinations
Gene Silencing
Humans
Image Processing, Computer-Assisted
Laminin
Male
Molecular Imaging
Neoplasm Invasiveness
Prostatic Neoplasms
Proteoglycans
RNA, Small Interfering
rho GTP-Binding Proteins
Journal Article

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title = "Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay",

abstract = "Rho GTPases are master regulators of cytoskeleton dynamics and therefore regulate cell motility. Rho GTPases, as well as their regulators and effectors, are often deregulated in cancers and thus contribute to tumor progression to metastasis. Cancer progression involves multiple steps, including invasion of the surrounding tissues. Several methods to investigate the invasion of tumors cells in 3D matrices in vitro have been developed. In this chapter we describe a 3D-based morphology assay that can be used for medium-throughput microscopy-based screening to identify regulators of cancer cell invasion. We use this method coupled to RNAi to investigate how Rho GTPases affect prostate cancer cell morphology in 3D Matrigel.",

keywords = "Cell Line, Tumor, Cell Movement, Cell Survival, Collagen, Drug Combinations, Gene Silencing, Humans, Image Processing, Computer-Assisted, Laminin, Male, Molecular Imaging, Neoplasm Invasiveness, Prostatic Neoplasms, Proteoglycans, RNA, Small Interfering, rho GTP-Binding Proteins, Journal Article",

author = "Audrey Colomba and Ridley, {Anne J}",

year = "2014",

doi = "10.1007/978-1-62703-791-4_21",

language = "English",

volume = "1120",

pages = "327--37",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay

AU - Colomba, Audrey

AU - Ridley, Anne J

PY - 2014

Y1 - 2014

N2 - Rho GTPases are master regulators of cytoskeleton dynamics and therefore regulate cell motility. Rho GTPases, as well as their regulators and effectors, are often deregulated in cancers and thus contribute to tumor progression to metastasis. Cancer progression involves multiple steps, including invasion of the surrounding tissues. Several methods to investigate the invasion of tumors cells in 3D matrices in vitro have been developed. In this chapter we describe a 3D-based morphology assay that can be used for medium-throughput microscopy-based screening to identify regulators of cancer cell invasion. We use this method coupled to RNAi to investigate how Rho GTPases affect prostate cancer cell morphology in 3D Matrigel.

AB - Rho GTPases are master regulators of cytoskeleton dynamics and therefore regulate cell motility. Rho GTPases, as well as their regulators and effectors, are often deregulated in cancers and thus contribute to tumor progression to metastasis. Cancer progression involves multiple steps, including invasion of the surrounding tissues. Several methods to investigate the invasion of tumors cells in 3D matrices in vitro have been developed. In this chapter we describe a 3D-based morphology assay that can be used for medium-throughput microscopy-based screening to identify regulators of cancer cell invasion. We use this method coupled to RNAi to investigate how Rho GTPases affect prostate cancer cell morphology in 3D Matrigel.

KW - Cell Line, Tumor

KW - Cell Movement

KW - Cell Survival

KW - Collagen

KW - Drug Combinations

KW - Gene Silencing

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Laminin

KW - Male

KW - Molecular Imaging

KW - Neoplasm Invasiveness

KW - Prostatic Neoplasms

KW - Proteoglycans

KW - RNA, Small Interfering

KW - rho GTP-Binding Proteins

KW - Journal Article

U2 - 10.1007/978-1-62703-791-4_21

DO - 10.1007/978-1-62703-791-4_21

M3 - Article (Academic Journal)

C2 - 24470035

VL - 1120

SP - 327

EP - 337

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay

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Keywords

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