Antimicrobial Resistance Patterns and Biofilms Resurgence Ability of Escherichia coli Associated with Commercial Layer Chicken Farms in Sri Lanka

Iresha Subhasinghe, H.M.H.N. Bandara*, H.M.T.K. Karunarathna, S.P. Kodithuwakku, H.C. Gallage, R.S. Kalupahana, K.S.A. Kottawatta*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

This study aims to understand antimicrobial resistance (AMR) associated with planktonic and biofilm-forming bacteria in Sri Lankan chicken industry. Planktonic or biofilm-forming Escherichia coli were isolated from different sources in layer chicken farms and their AMR profiles were determined. Minimum inhibitory concentrations (MICs) of selected antibiotics were quantified against planktonic E. coli isolated from water. Minimum biofilm inhibitory/eradication concentrations (MBIC/MBEC) of tetracycline were determined for biofilm E. coli. Faecal E. coli demonstrated highest resistance to tetracycline (64% of isolates). Biofilm-derived planktonic E. coli showed greater resistance to antibiotics than planktonic E. coli (75% vs 62.5%). The MBIC and MBEC of tetracycline in E. coli biofilm phenotype were significantly greater than MIC of planktonic phenotype of the same isolate (P
Original languageEnglish
Article number110422
Number of pages10
JournalVeterinary Microbiology
Volume302
Early online date5 Feb 2025
DOIs
Publication statusPublished - 1 Mar 2025

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