Antisense oligonucleotide against the Ca channel β subunit decreases L-type Ca current in rat ventricular myocytes

Rob N. Leach, Fabien Brette, Clive H. Orchard*

*Corresponding author for this work

    Research output: Contribution to journalArticle (Academic Journal)peer-review

    2 Citations (Scopus)

    Abstract

    The role of endogenous β subunits of the L-type Ca channel in native cardiac ventricular myocytes is unclear. We have therefore investigated the effect of inhibiting β subunit expression in rat myocytes, by culturing isolated myocytes for 24 h with either antisense oligonucleotide against the β subunit or with scrambled oligonucleotide (control). α1 subunit expression and distribution were then determined by immunolabeling, and L-type Ca current measured using the whole cell patch-clamp technique. Cells treated with antisense showed increased perinuclear staining for α1, decreased Ca current amplitude and a small rightward shift of the activation curve and the I-V relation, with no significant effect on inactivation. These data suggest that endogenous β subunits in native cardiac myocytes help to traffic the α1 subunit to the cell membrane and thus play a major role in determining Ca current amplitude.

    Original languageEnglish
    Pages (from-to)794-798
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume352
    Issue number3
    DOIs
    Publication statusPublished - 19 Jan 2007

    Keywords

    • β Subunit
    • Calcium current
    • Cardiac myocytes
    • L-type Ca channel

    Fingerprint Dive into the research topics of 'Antisense oligonucleotide against the Ca channel β subunit decreases L-type Ca current in rat ventricular myocytes'. Together they form a unique fingerprint.

    Cite this