ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells

Mariana Lazarini; Fabiola Traina; João A Machado-Neto; Karin S A Barcellos; Yuri B Moreira; Marcelo M Brandão; Sergio Verjovski-Almeida; Anne J Ridley; Sara T Olalla Saad

doi:10.1016/j.bbadis.2012.11.010

ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells

Mariana Lazarini, Fabiola Traina, João A Machado-Neto, Karin S A Barcellos, Yuri B Moreira, Marcelo M Brandão, Sergio Verjovski-Almeida, Anne J Ridley, Sara T Olalla Saad

Research output: Contribution to journal › Article (Academic Journal) › peer-review

43 Citations (Scopus)

Abstract

BACKGROUND: Several Rho GTPase-activating proteins (RhoGAPs) are implicated in tumor progression through their effects on Rho GTPase activity. ARHGAP21 is a RhoGAP with increased expression in head and neck squamous cell carcinoma and with a possible role in glioblastoma tumor progression, yet little is known about the function of ARHGAP21 in cancer cells. Here we studied the role of ARHGAP21 in two prostate adenocarcinoma cell lines, LNCaP and PC3, which respectively represent initial and advanced stages of prostate carcinogenesis.

RESULTS: ARHGAP21 is located in the nucleus and cytoplasm of both cell lines and its depletion resulted in decreased proliferation and increased migration of PC3 cells but not LNCaP cells. In PC3 cells, ARHGAP21 presented GAP activity for RhoA and RhoC and induced changes in cell morphology. Moreover, its silencing altered the expression of genes involved in cell proliferation and cytoskeleton organization, as well as the endothelin-1 canonical pathway.

CONCLUSIONS: Our results reveal new functions and signaling pathways regulated by ARHGAP21, and indicate that it could contribute to prostate cancer progression.

Original language	English
Pages (from-to)	365-74
Number of pages	10
Journal	Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Volume	1832
Issue number	2
DOIs	https://doi.org/10.1016/j.bbadis.2012.11.010
Publication status	Published - Feb 2013

Keywords

Adenocarcinoma
Base Sequence
Cell Line, Tumor
Cell Movement
Cell Proliferation
DNA Primers
GTPase-Activating Proteins
Gene Silencing
Humans
In Situ Nick-End Labeling
Male
Prostatic Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbadis.2012.11.010

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Lazarini, M., Traina, F., Machado-Neto, J. A., Barcellos, K. S. A., Moreira, Y. B., Brandão, M. M., Verjovski-Almeida, S., Ridley, A. J., & Saad, S. T. O. (2013). ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1832(2), 365-74. https://doi.org/10.1016/j.bbadis.2012.11.010

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title = "ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells",

abstract = "BACKGROUND: Several Rho GTPase-activating proteins (RhoGAPs) are implicated in tumor progression through their effects on Rho GTPase activity. ARHGAP21 is a RhoGAP with increased expression in head and neck squamous cell carcinoma and with a possible role in glioblastoma tumor progression, yet little is known about the function of ARHGAP21 in cancer cells. Here we studied the role of ARHGAP21 in two prostate adenocarcinoma cell lines, LNCaP and PC3, which respectively represent initial and advanced stages of prostate carcinogenesis.RESULTS: ARHGAP21 is located in the nucleus and cytoplasm of both cell lines and its depletion resulted in decreased proliferation and increased migration of PC3 cells but not LNCaP cells. In PC3 cells, ARHGAP21 presented GAP activity for RhoA and RhoC and induced changes in cell morphology. Moreover, its silencing altered the expression of genes involved in cell proliferation and cytoskeleton organization, as well as the endothelin-1 canonical pathway.CONCLUSIONS: Our results reveal new functions and signaling pathways regulated by ARHGAP21, and indicate that it could contribute to prostate cancer progression.",

keywords = "Adenocarcinoma, Base Sequence, Cell Line, Tumor, Cell Movement, Cell Proliferation, DNA Primers, GTPase-Activating Proteins, Gene Silencing, Humans, In Situ Nick-End Labeling, Male, Prostatic Neoplasms, Reverse Transcriptase Polymerase Chain Reaction, Journal Article, Research Support, Non-U.S. Gov't",

author = "Mariana Lazarini and Fabiola Traina and Machado-Neto, {Jo{\~a}o A} and Barcellos, {Karin S A} and Moreira, {Yuri B} and Brand{\~a}o, {Marcelo M} and Sergio Verjovski-Almeida and Ridley, {Anne J} and Saad, {Sara T Olalla}",

year = "2013",

month = feb,

doi = "10.1016/j.bbadis.2012.11.010",

language = "English",

volume = "1832",

pages = "365--74",

journal = "Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease",

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}

Lazarini, M, Traina, F, Machado-Neto, JA, Barcellos, KSA, Moreira, YB, Brandão, MM, Verjovski-Almeida, S, Ridley, AJ & Saad, STO 2013, 'ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells', Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, vol. 1832, no. 2, pp. 365-74. https://doi.org/10.1016/j.bbadis.2012.11.010

ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells. / Lazarini, Mariana; Traina, Fabiola; Machado-Neto, João A et al.
In: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Vol. 1832, No. 2, 02.2013, p. 365-74.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells

AU - Lazarini, Mariana

AU - Traina, Fabiola

AU - Machado-Neto, João A

AU - Barcellos, Karin S A

AU - Moreira, Yuri B

AU - Brandão, Marcelo M

AU - Verjovski-Almeida, Sergio

AU - Ridley, Anne J

AU - Saad, Sara T Olalla

PY - 2013/2

Y1 - 2013/2

N2 - BACKGROUND: Several Rho GTPase-activating proteins (RhoGAPs) are implicated in tumor progression through their effects on Rho GTPase activity. ARHGAP21 is a RhoGAP with increased expression in head and neck squamous cell carcinoma and with a possible role in glioblastoma tumor progression, yet little is known about the function of ARHGAP21 in cancer cells. Here we studied the role of ARHGAP21 in two prostate adenocarcinoma cell lines, LNCaP and PC3, which respectively represent initial and advanced stages of prostate carcinogenesis.RESULTS: ARHGAP21 is located in the nucleus and cytoplasm of both cell lines and its depletion resulted in decreased proliferation and increased migration of PC3 cells but not LNCaP cells. In PC3 cells, ARHGAP21 presented GAP activity for RhoA and RhoC and induced changes in cell morphology. Moreover, its silencing altered the expression of genes involved in cell proliferation and cytoskeleton organization, as well as the endothelin-1 canonical pathway.CONCLUSIONS: Our results reveal new functions and signaling pathways regulated by ARHGAP21, and indicate that it could contribute to prostate cancer progression.

AB - BACKGROUND: Several Rho GTPase-activating proteins (RhoGAPs) are implicated in tumor progression through their effects on Rho GTPase activity. ARHGAP21 is a RhoGAP with increased expression in head and neck squamous cell carcinoma and with a possible role in glioblastoma tumor progression, yet little is known about the function of ARHGAP21 in cancer cells. Here we studied the role of ARHGAP21 in two prostate adenocarcinoma cell lines, LNCaP and PC3, which respectively represent initial and advanced stages of prostate carcinogenesis.RESULTS: ARHGAP21 is located in the nucleus and cytoplasm of both cell lines and its depletion resulted in decreased proliferation and increased migration of PC3 cells but not LNCaP cells. In PC3 cells, ARHGAP21 presented GAP activity for RhoA and RhoC and induced changes in cell morphology. Moreover, its silencing altered the expression of genes involved in cell proliferation and cytoskeleton organization, as well as the endothelin-1 canonical pathway.CONCLUSIONS: Our results reveal new functions and signaling pathways regulated by ARHGAP21, and indicate that it could contribute to prostate cancer progression.

KW - Adenocarcinoma

KW - Base Sequence

KW - Cell Line, Tumor

KW - Cell Movement

KW - Cell Proliferation

KW - DNA Primers

KW - GTPase-Activating Proteins

KW - Gene Silencing

KW - Humans

KW - In Situ Nick-End Labeling

KW - Male

KW - Prostatic Neoplasms

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.bbadis.2012.11.010

DO - 10.1016/j.bbadis.2012.11.010

M3 - Article (Academic Journal)

C2 - 23200924

SN - 0925-4439

VL - 1832

SP - 365

EP - 374

JO - Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

JF - Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

IS - 2

ER -

ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells

Abstract

Keywords

UN SDGs

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