Artificial membrane-binding proteins stimulate oxygenation of stem cells during engineering of large cartilage tissue

James P K Armstrong, Rameen Shakur, Joseph P. Horne, Sally C. Dickinson, Craig T. Armstrong, Katherine Lau, Juned Kadiwala, Robert Lowe, Annela Seddon, Stephen Mann, J. L Ross Anderson, Adam W. Perriman*, Anthony P. Hollander

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

45 Citations (Scopus)

Abstract

Restricted oxygen diffusion can result in central cell necrosis in engineered tissue, a problem that is exacerbated when engineering large tissue constructs for clinical application. Here we show that pre-treating human mesenchymal stem cells (hMSCs) with synthetic membrane-active myoglobin-polymer-surfactant complexes can provide a reservoir of oxygen capable of alleviating necrosis at the centre of hyaline cartilage. This is achieved through the development of a new cell functionalization methodology based on polymer-surfactant conjugation, which allows the delivery of functional proteins to the hMSC membrane. This new approach circumvents the need for cell surface engineering using protein chimerization or genetic transfection, and we demonstrate that the surface-modified hMSCs retain their ability to proliferate and to undergo multilineage differentiation. The functionalization technology is facile, versatile and non-disruptive, and in addition to tissue oxygenation, it should have far-reaching application in a host of tissue engineering and cell-based therapies.

Original languageEnglish
Article number7405
Pages (from-to)7405
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 17 Jun 2015

Structured keywords

  • CRICBristol
  • Bristol BioDesign Institute

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