Aryl azide photochemistry in defined protein environments.

Josie L Morris; Samuel Reddington; Damien Murphy; D. Dafydd Jones; James Platts; Eric Tippmann

doi:10.1021/ol3028779

Aryl azide photochemistry in defined protein environments.

Josie L Morris, Samuel Reddington, Damien Murphy, D. Dafydd Jones, James Platts, Eric Tippmann

School of Biochemistry

Research output: Contribution to journal › Article (Academic Journal) › peer-review

15 Citations (Scopus)

Abstract

A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.

Original language	English
Pages (from-to)	728-731
Journal	Organic Letters
Volume	15
Issue number	4
DOIs	https://doi.org/10.1021/ol3028779
Publication status	Published - 25 Jan 2013

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title = "Aryl azide photochemistry in defined protein environments.",

abstract = "A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.",

author = "Morris, {Josie L} and Samuel Reddington and Damien Murphy and Jones, {D. Dafydd} and James Platts and Eric Tippmann",

year = "2013",

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T1 - Aryl azide photochemistry in defined protein environments.

AU - Morris, Josie L

AU - Reddington, Samuel

AU - Murphy, Damien

AU - Jones, D. Dafydd

AU - Platts, James

AU - Tippmann, Eric

PY - 2013/1/25

Y1 - 2013/1/25

N2 - A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.

AB - A genetically encoded precursor to an aryl nitrene, para-azidophenylalanine, was introduced site specifically into proteins to deduce if distinct environments were capable of caging a reactive organic intermediate. Following photolysis of mutant T4 lysozyme or green fluorescent proteins, EPR spectra showed, respectively, the presence of a triplet nitrene and an anilino radical.

U2 - 10.1021/ol3028779

DO - 10.1021/ol3028779

M3 - Article (Academic Journal)

C2 - 23351055

SN - 1523-7060

VL - 15

SP - 728

EP - 731

JO - Organic Letters

JF - Organic Letters

IS - 4

ER -

Aryl azide photochemistry in defined protein environments.

Abstract

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