Assays for transcription factor activity

D Browning, NJ Savery, A Kolb, S Busby

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

8 Citations (Scopus)


Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.
Translated title of the contributionAssays for transcription
Original languageEnglish
Title of host publicationMethods Molecular Biology
Subtitle of host publicationDNA-Protein Interactions
EditorsTom Moss, Benoit Leblanc
Place of PublicationDordrecht
PublisherSpringer Netherlands
Pages369 - 387
Number of pages19
ISBN (Electronic)978-1-60327-015-1
ISBN (Print)978-1-60327-014-4
Publication statusPublished - 2009


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