Abstract
Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.
Translated title of the contribution | Assays for transcription |
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Original language | English |
Title of host publication | Methods Molecular Biology |
Subtitle of host publication | DNA-Protein Interactions |
Editors | Tom Moss, Benoit Leblanc |
Place of Publication | Dordrecht |
Publisher | Springer Netherlands |
Pages | 369 - 387 |
Number of pages | 19 |
Edition | 3rd |
ISBN (Electronic) | 978-1-60327-015-1 |
ISBN (Print) | 978-1-60327-014-4 |
DOIs | |
Publication status | Published - 2009 |