Assays for transcription factor activity

D Browning; NJ Savery; A Kolb; S Busby

doi:10.1007/978-1-60327-015-1_23

Assays for transcription factor activity

D Browning
, NJ Savery
, A Kolb
, S Busby

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

8 Citations (Scopus)

Abstract

Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.

Translated title of the contribution	Assays for transcription
Original language	English
Title of host publication	Methods Molecular Biology
Subtitle of host publication	DNA-Protein Interactions
Editors	Tom Moss, Benoit Leblanc
Place of Publication	Dordrecht
Publisher	Springer Netherlands
Pages	369 - 387
Number of pages	19
Edition	3rd
ISBN (Electronic)	978-1-60327-015-1
ISBN (Print)	978-1-60327-014-4
DOIs	https://doi.org/10.1007/978-1-60327-015-1_23
Publication status	Published - 2009

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10.1007/978-1-60327-015-1_23

http://www.springerlink.com/content/k7775l2142462w87/#section=55382&page=1

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title = "Assays for transcription factor activity",

abstract = "Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.",

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T1 - Assays for transcription factor activity

AU - Browning, D

AU - Savery, NJ

AU - Kolb, A

AU - Busby, S

PY - 2009

Y1 - 2009

N2 - Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.

AB - Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, abortive initiation assays, and potassium permanganate footprinting. These techniques have been developed using bacterial systems, and can be used to study the kinetics of transcription initiation, and hence to unravel regulatory mechanisms.

U2 - 10.1007/978-1-60327-015-1_23

DO - 10.1007/978-1-60327-015-1_23

M3 - Chapter in a book

C2 - 19378177

SN - 978-1-60327-014-4

SP - 369

EP - 387

BT - Methods Molecular Biology

A2 - Moss, Tom

A2 - Leblanc, Benoit

PB - Springer Netherlands

CY - Dordrecht

ER -

Assays for transcription factor activity

Abstract

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