Assembly of Baculovirus Vectors for Multiplexed Prime Editing

Francesco Aulicino; Renata A Raele; Alexandra Harrison; Imre Berger

doi:10.1007/978-1-0716-3961-0_24

Assembly of Baculovirus Vectors for Multiplexed Prime Editing

Francesco Aulicino, Renata A Raele, Alexandra Harrison, Imre Berger^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter in a book

2 Citations (Scopus)

Abstract

Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to mammalian cells. Amongst all editing approaches, prime editing (PE) has the unique potential to perform seamless genome rewriting, in the absence of DNA double-strand breaks (DSBs). The cargo capacity required for efficient PE delivery to mammalian cells stands at odd with the limited packaging capacity of traditional viral delivery vectors. By contrast, baculovirus (BV) has a large synthetic DNA capacity and can efficiently transduce mammalian cells. Here we describe a protocol for the assembly of baculovirus vectors for multiplexed prime editing in mammalian cells.

Original language	English
Title of host publication	Baculovirus
Subtitle of host publication	Methods and Protocols
Publisher	Springer
Chapter	24
Pages	301-327
Number of pages	27
ISBN (Electronic)	9781071639610
ISBN (Print)	9781071639603
DOIs	https://doi.org/10.1007/978-1-0716-3961-0_24
Publication status	Published - 2 Jul 2024

Publication series

Name	Methods in Molecular Biology
Volume	2829
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Keywords

Baculoviridae/genetics
Gene Editing/methods
Genetic Vectors/genetics
CRISPR-Cas Systems
Humans
Animals
HEK293 Cells

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abstract = "Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to mammalian cells. Amongst all editing approaches, prime editing (PE) has the unique potential to perform seamless genome rewriting, in the absence of DNA double-strand breaks (DSBs). The cargo capacity required for efficient PE delivery to mammalian cells stands at odd with the limited packaging capacity of traditional viral delivery vectors. By contrast, baculovirus (BV) has a large synthetic DNA capacity and can efficiently transduce mammalian cells. Here we describe a protocol for the assembly of baculovirus vectors for multiplexed prime editing in mammalian cells.",

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T1 - Assembly of Baculovirus Vectors for Multiplexed Prime Editing

AU - Aulicino, Francesco

AU - Raele, Renata A

AU - Harrison, Alexandra

AU - Berger, Imre

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

PY - 2024/7/2

Y1 - 2024/7/2

N2 - Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to mammalian cells. Amongst all editing approaches, prime editing (PE) has the unique potential to perform seamless genome rewriting, in the absence of DNA double-strand breaks (DSBs). The cargo capacity required for efficient PE delivery to mammalian cells stands at odd with the limited packaging capacity of traditional viral delivery vectors. By contrast, baculovirus (BV) has a large synthetic DNA capacity and can efficiently transduce mammalian cells. Here we describe a protocol for the assembly of baculovirus vectors for multiplexed prime editing in mammalian cells.

AB - Efficient genome editing by using CRISPR technologies requires simultaneous and efficient delivery of multiple genetically encoded components to mammalian cells. Amongst all editing approaches, prime editing (PE) has the unique potential to perform seamless genome rewriting, in the absence of DNA double-strand breaks (DSBs). The cargo capacity required for efficient PE delivery to mammalian cells stands at odd with the limited packaging capacity of traditional viral delivery vectors. By contrast, baculovirus (BV) has a large synthetic DNA capacity and can efficiently transduce mammalian cells. Here we describe a protocol for the assembly of baculovirus vectors for multiplexed prime editing in mammalian cells.

KW - Baculoviridae/genetics

KW - Gene Editing/methods

KW - Genetic Vectors/genetics

KW - CRISPR-Cas Systems

KW - Humans

KW - Animals

KW - HEK293 Cells

U2 - 10.1007/978-1-0716-3961-0_24

DO - 10.1007/978-1-0716-3961-0_24

M3 - Chapter in a book

C2 - 38951346

SN - 9781071639603

T3 - Methods in Molecular Biology

SP - 301

EP - 327

BT - Baculovirus

PB - Springer

ER -

Assembly of Baculovirus Vectors for Multiplexed Prime Editing

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