Projects per year
Abstract
Summary Ionotropic glutamate receptor (iGluR) trafficking and function underpin excitatory synaptic transmission and plasticity and shape neuronal networks. It is well established that the transcription, translation, and endocytosis/recycling of iGluRs are all regulated by neuronal activity, but much less is known about the activity dependence of iGluR transport through the secretory pathway. Here, we use the kainate receptor subunit GluK2 as a model iGluR cargo to show that the assembly, early secretory pathway trafficking, and surface delivery of iGluRs are all controlled by neuronal activity. We show that the delivery of de novo kainate receptors is differentially regulated by modulation of GluK2 Q/R editing, PKC phosphorylation, and PDZ ligand interactions. These findings reveal that, in addition to short-term regulation of iGluRs by recycling/endocytosis and long-term modulation by altered transcription/translation, the trafficking of iGluRs through the secretory pathway is under tight activity-dependent control to determine the numbers and properties of surface-expressed iGluRs.
Original language | English |
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Pages (from-to) | 2613-2626 |
Number of pages | 14 |
Journal | Cell Reports |
Volume | 19 |
Issue number | 12 |
Early online date | 20 Jun 2017 |
DOIs | |
Publication status | Published - 20 Jun 2017 |
Keywords
- kainate receptor
- retention using selective hooks
- RUSH
- secretory pathway
- ER exit sites
- Golgi outposts
- AMPA receptors
- scaling
- Q/R editing
- ADAR2
- PDZ ligand
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Dive into the research topics of 'Assembly, Secretory Pathway Trafficking, and Surface Delivery of Kainate Receptors Is Regulated by Neuronal Activity'. Together they form a unique fingerprint.Projects
- 4 Finished
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Targets and mechanisms of SUMOylation-mediated cardioprotection during ischemia and reperfusion injury
Henley, J. M. (Principal Investigator)
1/04/15 → 3/10/19
Project: Research
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Roles of protein SUMOylation in AMPA receptor trafficking, synaptic dysfunction and cognitive impairment in dementia
Henley, J. M. (Principal Investigator)
1/03/14 → 30/06/18
Project: Research
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MECHANISMS CONTROLLING THE NUMBER AND LOCATION OF SYNAPTIC AMPR'S
Henley, J. M. (Principal Investigator)
1/01/08 → 1/01/13
Project: Research
Equipment
Profiles
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Emeritus Professor Jeremy M Henley
- School of Biochemistry - Emeritus Professor
- Dynamic Cell Biology
- Bristol Neuroscience
Person: Member, Honorary and Visiting Academic