Assessing the Stain Removal Potential of Experimental and Commercial Toothpastes

Jelena Taylor, Emma Macdonald, Nicola West

Research output: Contribution to conferenceConference Posterpeer-review


Objectives: The cosmetic appearance of teeth has become of importance to individuals, as can be shown by an increase in the development and sales of tooth-whitening kits. The aim of this in vitro study was to see how effective test toothpaste formulations are compared to commercially available pastes and water at removing extrinsic dietary stain. Experimental: Perspex samples of size 30x10x2mm were stained prior to exposure to 15 test products. This was achieved by sequential soaking in human stimulated whole saliva, sourced through an approved saliva bank, for 2 minutes. Followed by soaking in 10ml of 0.2% chlorhexidine based mouthwash for 2 minutes, then immersed in 10ml tea solution for 60 minutes. This cycle was repeated until measurements using a UV/VIS spectrophotometer, set at 395nm, reached an absorbance reading of ≥2.000. The stain removal treatment regimen involved cyclical exposure of the Perspex samples to 10ml of the toothpaste slurries, plus a water control for 5 minutes. Results: After 5 cycles the greatest degree of stain removal was from the 3 Beverly Hills Formula experimental toothpaste formulations, at 84.2%, 83.7% and 76.0% stain removal. From the commercially available toothpastes, Beverly Hills Formula Perfect White Black and Perfect White showed the greatest amount of stain removal at 66.7% and 63.5% respectively. As expected Deionised Water showed the least amount of stain removal at 13.2%, followed by Curaprox Black is White at 22.1% and Colgate Sensitive Pro Relief & Whitening at 30.5%, both of which were not significantly different to the amount removed by water. All other experimental and commercially available toothpastes showed a significant amount of stain removal compared to deionised water where p=0.05. Conclusion: All toothpastes showed a progressive degree of stain removal following each treatment cycle with three of the five experimental toothpastes showing the greatest amount of stain removal.
Original languageEnglish
Publication statusAccepted/In press - 4 Apr 2018
Event96th General Session of the IADR, 2018 - Excel London Convention Centre, London, United Kingdom
Duration: 25 Jul 201828 Jul 2018
Conference number: 96


Conference96th General Session of the IADR, 2018
Country/TerritoryUnited Kingdom
Internet address


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