Assessment and in vivo scoring of murine experimental autoimmune uveoretinitis using optical coherence tomography

Colin Chu, Philipp Herrmann, Livia S Carvalho, Sidath E Liyanage, James W B Bainbridge, Robin R Ali, Andrew D Dick, Ulrich F O Luhmann

Research output: Contribution to journalArticle (Academic Journal)peer-review

40 Citations (Scopus)
242 Downloads (Pure)

Abstract

Despite advances in clinical imaging and grading our understanding of retinal immune responses and their morphological correlates in experimental autoimmune uveoretinitis (EAU), has been hindered by the requirement for post-mortem histology. To date, monitoring changes occurring during EAU disease progression and evaluating the effect of therapeutic intervention in real time has not been possible. We wanted to establish whether optical coherence tomography (OCT) could detect intraretinal changes during inflammation and to determine its utility as a tool for accurate scoring of EAU. EAU was induced in C57BL/6J mice and animals evaluated after 15, 26, 36 and 60 days. At each time-point, contemporaneous Spectralis-OCT scanning, topical endoscopic fundal imaging (TEFI), fundus fluorescein angiography (FFA) and CD45-immunolabelled histology were performed. OCT features were further characterised on retinal flat-mounts using immunohistochemistry and 3D reconstruction. Optic disc swelling and vitreous opacities detected by OCT corresponded to CD45+ cell infiltration on histology. Vasculitis identified by FFA and OCT matched perivascular myeloid and T-cell infiltrates and could be differentiated from unaffected vessels. Evolution of these changes could be followed over time in the same eye. Retinal folds were visible and found to encapsulate mixed populations of activated myeloid cells, T-cells and microglia. Using these features, an OCT-based EAU scoring system was developed, with significant correlation to validated histological (Pearson r(2) = 0.6392, P
Original languageEnglish
Article numbere63002
Number of pages10
JournalPLoS ONE
Volume8
Issue number5
DOIs
Publication statusPublished - 14 May 2013

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