Assessment of plasma acylcarnitines before and after weight loss in obese subjects

Marieke G Schooneman, Antonella Napolitano, Sander M Houten, Graeme K Ambler, Peter R Murgatroyd, Sam R Miller, Carla E M Hollak, Chong Y Tan, Samuel Virtue, Antonio Vidal-Puig, Derek J Nunez, Maarten R Soeters

Research output: Contribution to journalArticle (Academic Journal)peer-review

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Abstract

Acylcarnitines, fatty acid oxidation (FAO) intermediates, have been implicated in diet-induced insulin resistance and type 2 diabetes mellitus, as increased levels are found in obese insulin resistant humans. Moreover plasma acylcarnitines have been associated with clinical parameters related to glucose metabolism, such as fasting glucose levels and HbA1c. We hypothesized that plasma acylcarnitines would correlate with energy expenditure, insulin sensitivity and other clinical parameters before and during a weight loss intervention. We measured plasma acylcarnitines in 60 obese subjects before and after a 12 week weight loss intervention. These samples originated from three different interventions (diet alone (n = 20); diet and exercise (n = 21); diet and drug treatment (n = 19)). Acylcarnitine profiles were analysed in relation to clinical parameters of glucose metabolism, insulin sensitivity and energy expenditure. Conclusions were drawn from all 60 subjects together. Despite amelioration of HOMA-IR, plasma acylcarnitines levels increased during weight loss. HOMA-IR, energy expenditure and respiratory exchange ratio were not related to plasma acylcarnitines. However non-esterified fatty acids correlated strongly with several acylcarnitines at baseline and during the weight loss intervention (p < 0.001). Acylcarnitines did not correlate with clinical parameters of glucose metabolism during weight loss, questioning their role in insulin resistance and type 2 diabetes mellitus.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume606
Early online date19 Jul 2016
DOIs
Publication statusPublished - 15 Sep 2016

Keywords

  • Adult
  • Anthropometry
  • Body Composition
  • Carnitine/analogs & derivatives
  • Diabetes Mellitus, Type 2/blood
  • Fatty Acids/chemistry
  • Fatty Acids, Nonesterified/blood
  • Female
  • Glucose/chemistry
  • Humans
  • Insulin Resistance
  • Lipolysis
  • Male
  • Middle Aged
  • Obesity/blood
  • Oxygen/chemistry
  • Respiration
  • Weight Loss
  • Young Adult

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