Abstract

A 7-year-old female neutered Jack Russell Terrier was presented to Langford Vets, the University of Bristol, with a history of chronic intermittent lethargy. Investigations and clinical course were compatible with hereditary hemolysis due to a red blood cell membrane defect. Proteomics was used to explore protein alterations in the presence of a hypothesized red blood cell membrane protein deficiency. Proteomic analysis revealed downregulation of the band 3, and alpha- and beta-adducin proteins, and alterations in the red blood cell proteome consistent with previous reports of changes due to the presence of reticulocytosis and ongoing hemolysis. The spectrum of protein alterations identified in the affected dog may be homologous to a band 3 protein deficiency secondary to hereditary spherocytosis, as described in people.

Original languageEnglish
Pages (from-to)377-385
Number of pages9
JournalVeterinary Clinical Pathology
Volume47
Issue number3
Early online date19 Jul 2018
DOIs
Publication statusPublished - 1 Sep 2018

Keywords

  • Dog
  • hemolysis
  • membrane
  • osmotic fragility
  • proteomics
  • red blood cell

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  • Equipment

    Proteomics Facility

    Kate Heesom (Manager)

    Faculty of Life Sciences

    Facility/equipment: Facility

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