Astrocytes Control Breathing Through pH-Dependent Release of ATP

AV Gourine, V Kasymov, N Marina, F Tang, MF Figueiredo, SL Lane, AG Teschemacher, KM Spyer, K Deisseroth, S Kasparov

Research output: Contribution to journalArticle (Academic Journal)

515 Citations (Scopus)

Abstract

Astrocytes provide structural and metabolic support for neuronal networks, but direct evidence demonstrating their active role in complex behaviors is limited. Central respiratory chemosensitivity is an essential mechanism that, via regulation of breathing, maintains constant levels of blood and brain pH and partial pressure of CO2. We found that astrocytes of the brainstem chemoreceptor areas are highly chemosensitive. They responded to physiological decreases in pH with vigorous elevations in intracellular Ca2+ and release of adenosine triphosphate (ATP). ATP propagated astrocytic Ca2+ excitation, activated chemoreceptor neurons, and induced adaptive increases in breathing. Mimicking pH-evoked Ca2+ responses by means of optogenetic stimulation of astrocytes expressing channelrhodopsin-2 activated chemoreceptor neurons via an ATP-dependent mechanism and triggered robust respiratory responses in vivo. This demonstrates a potentially crucial role for brain glial cells in mediating a fundamental physiological reflex
Translated title of the contributionAstrocytes Control Breathing Through pH-Dependent Release of ATP
Original languageEnglish
Pages (from-to)571 - 575
Number of pages5
JournalScience
Volume329
Early online date15 Jul 2010
DOIs
Publication statusPublished - 30 Jul 2010

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    Gourine, AV., Kasymov, V., Marina, N., Tang, F., Figueiredo, MF., Lane, SL., Teschemacher, AG., Spyer, KM., Deisseroth, K., & Kasparov, S. (2010). Astrocytes Control Breathing Through pH-Dependent Release of ATP. Science, 329, 571 - 575. https://doi.org/10.1126/science.1190721