Atomic Force Microscopy Reveals Drebrin Induced Remodeling of F-Actin with Subnanometer Resolution

Shivani Sharma*, Elena E. Grintsevich, Martin L. Phillips, Emil Reisler, James K. Gimzewski

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

69 Citations (Scopus)

Abstract

We show by high-resolution atomic force microscopy analysis that drebrin A (a major neuronal actin binding protein) induced F-actin structural and mechanical remodeling involves significant changes in helical twist and filament stiffness (+55% persistence length). These results provide evidence of a unique mechanical role of drebrin in the dendrites, contribute to current molecular-level understanding of the properties of the neuronal cytoskeleton, and reflect the role of biomechanics at the nanoscale, to modulate nanofilament-structure assemblies such as F-actin.

Original languageEnglish
Pages (from-to)825-827
Number of pages3
JournalNano Letters
Volume11
Issue number2
DOIs
Publication statusPublished - Feb 2011

Keywords

  • F-actin remodeling
  • drebrin
  • AFM
  • neuron cytoskeleton
  • nanofilament mechanics
  • FILAMENTS
  • FLEXIBILITY
  • DISRUPTION
  • DISORDERS
  • DYNAMICS
  • BINDING
  • PROTEIN
  • MODEL

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