Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites

David W. Green, Benjamin J. R. F. Bolland, Janos M. Kanczler, Stuart A. Lanham, Dominic Walsh, Stephen Mann, Richard O. C. Oreffo*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

31 Citations (Scopus)

Abstract

The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues. (C) 2008 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1918-1927
Number of pages10
JournalBiomaterials
Volume30
Issue number10
DOIs
Publication statusPublished - Apr 2009

Keywords

  • Human osteoprogenitor
  • Calcium carbonate
  • Osteogenesis
  • Biomimetic material
  • Mineralization
  • Bone tissue engineering
  • MARROW STROMAL CELLS
  • HYDROXYAPATITE MICROSPHERES
  • BIOMEDICAL APPLICATIONS
  • COMPOSITE MICROSPHERES
  • IN-VITRO
  • REPLACEMENT
  • SCAFFOLDS
  • DELIVERY
  • CULTURE
  • GROWTH

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