Automated 24-hours sampling of subcutaneous tissue free cortisol in humans

R C Bhake, J A Leendertz, A C E Linthorst, S L Lightman

Research output: Contribution to journalArticle (Academic Journal)peer-review

43 Citations (Scopus)


Hormonal systems are major regulators of metabolic and cognitive function and many of these, including the critical stress-responsive hypothalamic-pituitary-adrenal (HPA) axis, release their constituent hormones in a circadian manner. This circadian rhythmicity is made up from an underlying approximate hourly ultradian rhythm. In order to understand the importance of both circadian and ultradian rhythms in man it is important to be able to carry out multiple sampling studies over extended periods in a subject's home setting, which is the most meaningful physiological setting for homeostatically important hormones. This study has developed a novel automated sampling system that, when used in combination with a microdialysis system, collects timed samples of microdialysis fluid over a full 24 h in individuals going about their normal everyday activity. The apparatus has the capacity to provide sufficient sample volumes to measure changes in hormone concentration over 24 h, including the important period when subjects are asleep.
Original languageEnglish
Pages (from-to)180-4
Number of pages5
JournalJournal of Medical Engineering and Technology
Issue number3
Publication statusPublished - Apr 2013


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