Abstract
Structural and functional studies of many multiprotein complexes depend on recombinant-protein overexpression. Rapid revision of expression experiments and diversification of the complexes are often crucial for success of these projects; therefore, automation is increasingly indispensable. We introduce Acembl, a versatile and automatable system for protein-complex expression in Escherichia coli that uses recombineering to facilitate multigene assembly and diversification. We demonstrated protein-complex expression using Acembl, including production of the complete prokaryotic holotranslocon.
Original language | English |
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Pages (from-to) | 447 - 450 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 6 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 2009 |
Bibliographical note
Other: First published online 3rd May 2009Keywords
- Escherichia coli
- Multigene Family
- Protein Engineering
- Recombinant Proteins