Bacterial associations with weathering minerals at the regolith-bedrock interface, Luquillo Experimental Forest, Puerto Rico

Morgan Minyard; Mary Ann Bruns; Laura J. Liermann; Heather L Buss; Susan L. Brantley

doi:10.1080/01490451.2011.619640

Bacterial associations with weathering minerals at the regolith-bedrock interface, Luquillo Experimental Forest, Puerto Rico

Morgan Minyard, Mary Ann Bruns, Laura J. Liermann, Heather L Buss, Susan L. Brantley

Research output: Contribution to journal › Article (Academic Journal) › peer-review

25 Citations (Scopus)

519 Downloads (Pure)

Abstract

Microbe-mineral associations in regolith overlying granodiorite bedrock (4.6–4.9 m depth) from the Luquillo Experimental Forest, Puerto Rico, were imaged with confocal scanning laser microscopy at a novel scale of 400X magnification. After adding BacLight™ stain, proportionally more surface area of minerals (quartz, biotite, and mixed opaque kaolinite/goethite) emitted fluorescence from cell-impermeant propidium iodide than from cell-permeant SYTO 9, which suggested greater coverage of minerals by extracellular DNA or DNA in non-intact cells than by intact cells. Microscopic observations of predominantly non-intact cell material in deep saprolite were consistent with the abundance of rRNA sequences related to heterotrophic bacteria in clone libraries prepared from community DNA. A few sequences were affiliated with bacteria recognized to produce siderophores, oxidize Fe(II), or fix N2. Bacterial DNA in deep regolith from two boreholes 1.5 m apart yielded libraries with high diversity and taxa specific for each borehole. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the free supplemental files.

Translated title of the contribution	Bacterial associations with weathering minerals at the regolith-bedrock interface, Luquillo Experimental Forest, Puerto Rico
Original language	English
Pages (from-to)	792-803
Number of pages	12
Journal	Geomicrobiology Journal
Volume	29
Issue number	9
Early online date	1 May 2012
DOIs	https://doi.org/10.1080/01490451.2011.619640
Publication status	Published - 1 May 2012

Bibliographical note

Date of Acceptance: 18/08/2011

Keywords

regolith
confocal microscopy
iron oxidation
saprolite
16S rRNA

Access to Document

10.1080/01490451.2011.619640

Bruns_et_al_Geomicro_2011Accepted author manuscript, 1.7 MB

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Persistent link

Incipient chemical weathering at bedrock fracture interfaces in a tropical critical zone system, Puerto Rico
Moore, O. W., Buss, H. L. & Dosseto, A., May 2019, In: Geochimica et Cosmochimica Acta. 252, p. 61-87 27 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
File
20 Citations (Scopus)

153 Downloads (Pure)
Microbial chemolithotrophy mediates oxidative weathering of granitic bedrock
Napieralski, S. A., Buss, H. L., Brantley, S. L., Lee, S., Xu, H. & Roden, E. E., 26 Dec 2019, In: Proceedings of the National Academy of Sciences of the United States of America. 116, 52, p. 26394-26401 8 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

Open Access
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41 Citations (Scopus)

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Lithological influences on contemporary and long-term regolith weathering at the Luquillo Critical Zone Observatory
Buss, H. L., Chapela Lara, M., Moore, O. W., Kurtz, A. C., Schulz, M. S. & White, A. F., 1 Jan 2017, In: Geochimica et Cosmochimica Acta. 196, p. 224-251 28 p.
Research output: Contribution to journal › Article (Academic Journal) › peer-review

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74 Citations (Scopus)

457 Downloads (Pure)

1 Finished
1 Active

WEBB: USGS Water Energy and Biogeochemical Budgets
Buss, H. L. (Co-Principal Investigator), Scholl, M. (Researcher), White, A. F. (Researcher) & Shanley, J. (Co-Principal Investigator)
1/10/91 → …
Project: Research

File
LCZO: Luquillo Critical Zone Observatory
Buss, H. L. (Co-Investigator), Brantley, S. L. (Co-Investigator), Scatena, F. N. (Principal Investigator), Silver, W. (Co-Investigator), Plante, A. (Principal Investigator), Willenbring, J. (Co-Investigator), McDowell, W. (Principal Investigator), Shanley, J. (Co-Investigator), White, A. F. (Other ), Scholl, M. (Co-Investigator), Johnson, A. (Co-Investigator), Jerolmack, D. (Co-Investigator), Horton, B. (Co-Investigator), Chapela Lara, M. (Student) & Moore, O. W. (Student)
1/10/10 → 30/09/13
Project: Research

Cite this

@article{c09e271c51ac495a859b632251fe5ddf,

title = "Bacterial associations with weathering minerals at the regolith-bedrock interface, Luquillo Experimental Forest, Puerto Rico",

abstract = "Microbe-mineral associations in regolith overlying granodiorite bedrock (4.6–4.9 m depth) from the Luquillo Experimental Forest, Puerto Rico, were imaged with confocal scanning laser microscopy at a novel scale of 400X magnification. After adding BacLight{\texttrademark} stain, proportionally more surface area of minerals (quartz, biotite, and mixed opaque kaolinite/goethite) emitted fluorescence from cell-impermeant propidium iodide than from cell-permeant SYTO 9, which suggested greater coverage of minerals by extracellular DNA or DNA in non-intact cells than by intact cells. Microscopic observations of predominantly non-intact cell material in deep saprolite were consistent with the abundance of rRNA sequences related to heterotrophic bacteria in clone libraries prepared from community DNA. A few sequences were affiliated with bacteria recognized to produce siderophores, oxidize Fe(II), or fix N2. Bacterial DNA in deep regolith from two boreholes 1.5 m apart yielded libraries with high diversity and taxa specific for each borehole. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the free supplemental files.",

keywords = "regolith, confocal microscopy, iron oxidation, saprolite, 16S rRNA",

author = "Morgan Minyard and Bruns, \{Mary Ann\} and Liermann, \{Laura J.\} and Buss, \{Heather L\} and Brantley, \{Susan L.\}",

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AU - Minyard, Morgan

AU - Bruns, Mary Ann

AU - Liermann, Laura J.

AU - Buss, Heather L

AU - Brantley, Susan L.

N1 - Date of Acceptance: 18/08/2011

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N2 - Microbe-mineral associations in regolith overlying granodiorite bedrock (4.6–4.9 m depth) from the Luquillo Experimental Forest, Puerto Rico, were imaged with confocal scanning laser microscopy at a novel scale of 400X magnification. After adding BacLight™ stain, proportionally more surface area of minerals (quartz, biotite, and mixed opaque kaolinite/goethite) emitted fluorescence from cell-impermeant propidium iodide than from cell-permeant SYTO 9, which suggested greater coverage of minerals by extracellular DNA or DNA in non-intact cells than by intact cells. Microscopic observations of predominantly non-intact cell material in deep saprolite were consistent with the abundance of rRNA sequences related to heterotrophic bacteria in clone libraries prepared from community DNA. A few sequences were affiliated with bacteria recognized to produce siderophores, oxidize Fe(II), or fix N2. Bacterial DNA in deep regolith from two boreholes 1.5 m apart yielded libraries with high diversity and taxa specific for each borehole. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the free supplemental files.

AB - Microbe-mineral associations in regolith overlying granodiorite bedrock (4.6–4.9 m depth) from the Luquillo Experimental Forest, Puerto Rico, were imaged with confocal scanning laser microscopy at a novel scale of 400X magnification. After adding BacLight™ stain, proportionally more surface area of minerals (quartz, biotite, and mixed opaque kaolinite/goethite) emitted fluorescence from cell-impermeant propidium iodide than from cell-permeant SYTO 9, which suggested greater coverage of minerals by extracellular DNA or DNA in non-intact cells than by intact cells. Microscopic observations of predominantly non-intact cell material in deep saprolite were consistent with the abundance of rRNA sequences related to heterotrophic bacteria in clone libraries prepared from community DNA. A few sequences were affiliated with bacteria recognized to produce siderophores, oxidize Fe(II), or fix N2. Bacterial DNA in deep regolith from two boreholes 1.5 m apart yielded libraries with high diversity and taxa specific for each borehole. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the free supplemental files.

KW - regolith

KW - confocal microscopy

KW - iron oxidation

KW - saprolite

KW - 16S rRNA

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DO - 10.1080/01490451.2011.619640

M3 - Article (Academic Journal)

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JO - Geomicrobiology Journal

JF - Geomicrobiology Journal

IS - 9

ER -

Bacterial associations with weathering minerals at the regolith-bedrock interface, Luquillo Experimental Forest, Puerto Rico

Abstract

Bibliographical note

Keywords

Access to Document

Handle.net

Fingerprint

Research output

Incipient chemical weathering at bedrock fracture interfaces in a tropical critical zone system, Puerto Rico

Microbial chemolithotrophy mediates oxidative weathering of granitic bedrock

Lithological influences on contemporary and long-term regolith weathering at the Luquillo Critical Zone Observatory

Projects

WEBB: USGS Water Energy and Biogeochemical Budgets

LCZO: Luquillo Critical Zone Observatory

Cite this