Bacterial Reaction Centers Purified with Styrene Maleic Acid Copolymer Retain Native Membrane Functional Properties and Display Enhanced Stability

David J K Swainsbury, Stefan Scheidelaar, Rienk van Grondelle, J Antoinette Killian, Michael R Jones

Research output: Contribution to journalArticle (Academic Journal)peer-review

93 Citations (Scopus)

Abstract

Integral membrane proteins often present daunting challenges for biophysical characterization, a fundamental issue being how to select a surfactant that will optimally preserve the individual structure and functional properties of a given membrane protein. Bacterial reaction centers offer a rare opportunity to compare the properties of an integral membrane protein in different artificial lipid/surfactant environments with those in the native bilayer. Here, we demonstrate that reaction centers purified using a styrene maleic acid copolymer remain associated with a complement of native lipids and do not display the modified functional properties that typically result from detergent solubilization. Direct comparisons show that reaction centers are more stable in this copolymer/lipid environment than in a detergent micelle or even in the native membrane, suggesting a promising new route to exploitation of such photovoltaic integral membrane proteins in device applications.

Original languageEnglish
Pages (from-to)11803-11807
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number44
Early online date21 Oct 2014
DOIs
Publication statusPublished - 27 Oct 2014

Bibliographical note

© 2014 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Fingerprint Dive into the research topics of 'Bacterial Reaction Centers Purified with Styrene Maleic Acid Copolymer Retain Native Membrane Functional Properties and Display Enhanced Stability'. Together they form a unique fingerprint.

Cite this