Projects per year
Abstract
Motivation
In experimental protein engineering, alanine-scanning mutagenesis involves the replacement of selected residues with alanine to determine the energetic contribution of each side chain to forming an interaction. For example, it is often used to study protein-protein interactions. However, such experiments can be time-consuming and costly, which has led to the development of programs for performing computational alanine-scanning mutagenesis (CASM) to guide experiments. While programs are available for this, there is a need for a real-time web application that is accessible to non-expert users.
In experimental protein engineering, alanine-scanning mutagenesis involves the replacement of selected residues with alanine to determine the energetic contribution of each side chain to forming an interaction. For example, it is often used to study protein-protein interactions. However, such experiments can be time-consuming and costly, which has led to the development of programs for performing computational alanine-scanning mutagenesis (CASM) to guide experiments. While programs are available for this, there is a need for a real-time web application that is accessible to non-expert users.
| Original language | English |
|---|---|
| Article number | btaa026 |
| Number of pages | 3 |
| Journal | Bioinformatics |
| Early online date | 13 Jan 2020 |
| DOIs | |
| Publication status | E-pub ahead of print - 13 Jan 2020 |
Structured keywords
- Bristol BioDesign Institute
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- 2 Active
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Rational computational protein design in ISAMBARD: new approaches, folds and functions
1/05/18 → 30/04/23
Project: Research
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