Barcoding in trypanosomes

Rachel Hutchinson, Jamie Stevens

Research output: Contribution to journalReview article (Academic Journal)peer-review

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Abstract

Trypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of different barcoding techniques and examine the requirements of the ‘ideal’ trypanosome genetic barcode. In addition, we explore potential alternative genetic markers for barcoding Trypanosoma species, including an analysis of phylogenetically informative nucleotide changes along the length of the 18S rRNA gene.
Original languageEnglish
JournalParasitology
Early online date23 Nov 2017
DOIs
Publication statusE-pub ahead of print - 23 Nov 2017

Keywords

  • 18S rRNA
  • Chagas disease
  • cytochrome oxidase I
  • molecular methods
  • sleeping sickness
  • Trypanosoma
  • Trypanosomatidae

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