Barcoding in trypanosomes

Rachel Hutchinson; Jamie Stevens

doi:10.1017/S0031182017002049

Barcoding in trypanosomes

Rachel Hutchinson, Jamie Stevens

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Abstract

Trypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of different barcoding techniques and examine the requirements of the ‘ideal’ trypanosome genetic barcode. In addition, we explore potential alternative genetic markers for barcoding Trypanosoma species, including an analysis of phylogenetically informative nucleotide changes along the length of the 18S rRNA gene.

Original language	English
Journal	Parasitology
Early online date	23 Nov 2017
DOIs	https://doi.org/10.1017/S0031182017002049
Publication status	E-pub ahead of print - 23 Nov 2017

Keywords

18S rRNA
Chagas disease
cytochrome oxidase I
molecular methods
sleeping sickness
Trypanosoma
Trypanosomatidae

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10.1017/S0031182017002049

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This is the final published version of the article (version of record). It first appeared online via Cambridge CORE at https://www.cambridge.org/core/journals/parasitology/article/barcoding-in-trypanosomes/70EC549E55ED31D877EC46A22994F275/core-reader. Please refer to any applicable terms of use of the publisher.
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title = "Barcoding in trypanosomes",

abstract = "Trypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of different barcoding techniques and examine the requirements of the {\textquoteleft}ideal{\textquoteright} trypanosome genetic barcode. In addition, we explore potential alternative genetic markers for barcoding Trypanosoma species, including an analysis of phylogenetically informative nucleotide changes along the length of the 18S rRNA gene.",

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language = "English",

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AU - Hutchinson, Rachel

AU - Stevens, Jamie

PY - 2017/11/23

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N2 - Trypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of different barcoding techniques and examine the requirements of the ‘ideal’ trypanosome genetic barcode. In addition, we explore potential alternative genetic markers for barcoding Trypanosoma species, including an analysis of phylogenetically informative nucleotide changes along the length of the 18S rRNA gene.

AB - Trypanosomes (genus Trypanosoma) are parasites of humans, and wild and domestic mammals, in which they cause several economically and socially important diseases, including sleeping sickness in Africa and Chagas disease in the Americas. Despite the development of numerous molecular diagnostics and increasing awareness of the importance of these neglected parasites, there is currently no universal genetic barcoding marker available for trypanosomes. In this review we provide an overview of the methods used for trypanosome detection and identification, discuss the potential application of different barcoding techniques and examine the requirements of the ‘ideal’ trypanosome genetic barcode. In addition, we explore potential alternative genetic markers for barcoding Trypanosoma species, including an analysis of phylogenetically informative nucleotide changes along the length of the 18S rRNA gene.

KW - 18S rRNA

KW - Chagas disease

KW - cytochrome oxidase I

KW - molecular methods

KW - sleeping sickness

KW - Trypanosoma

KW - Trypanosomatidae

UR - https://www.cambridge.org/core/journals/parasitology/article/barcoding-in-trypanosomes/70EC549E55ED31D877EC46A22994F275/share/6fad97cefab6563931051c8bf5a58aa0490cc719

U2 - 10.1017/S0031182017002049

DO - 10.1017/S0031182017002049

M3 - Review article (Academic Journal)

C2 - 29168449

JO - Parasitology

JF - Parasitology

SN - 0031-1820

ER -

Barcoding in trypanosomes

Abstract

Keywords

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