Basigin mediation of  Plasmodium falciparum red blood cell invasion does not require its transmembrane domain or interaction with monocarboxylate transporter 1

Nadine R King, Catarina Martins Freire, Jawida Touhami, Marc Sitbon, Ashley M Toye, Timothy J Satchwell

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)

Abstract

Plasmodium falciparum invasion of the red blood cell is reliant upon the essential interaction of PfRh5 with the host receptor protein basigin. Basigin exists as part of one or more multiprotein complexes, most notably through interaction with the monocarboxylate transporter MCT1. However, the potential requirement for basigin association with MCT1 and the wider role of basigin host membrane context and lateral protein associations during merozoite invasion has not been established. Using genetically manipulated in vitro derived reticulocytes, we demonstrate the ability to uncouple basigin ectodomain presentation from its transmembrane domain-mediated interactions, including with MCT1. Merozoite invasion of reticulocytes is unaffected by disruption of basigin-MCT1 interaction and by removal or replacement of the basigin transmembrane helix. Therefore, presentation of the basigin ectodomain at the red blood cell surface, independent of its native association with MCT1 or other interactions mediated by the transmembrane domain, is sufficient to facilitate merozoite invasion.

Original languageEnglish
Article numbere1011989
JournalPLoS Pathogens
Volume20
Issue number2
Early online date5 Feb 2024
DOIs
Publication statusE-pub ahead of print - 5 Feb 2024

Bibliographical note

Funding Information:
Funding:Thisstudywassupportedthrough fundingprovidedbytheMedicalResearchCouncil (MR/V010506/1)toAMTandTJSandthe EuropeanUnionITN‘EVIDENCE’grantagreement ID860436andinpartbyfundingfromFondation pourlaRechercheMe ´ dicale(FRM)grant DBI201312285579(toMS)andNationalInstitutes ofHealth(NIH)grantDK32094(toMS).The fundersplayednoroleinthestudydesign,data collectionandanalysis,decisiontopublishor preparationofthemanuscript.

Publisher Copyright:
© 2024 King et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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