Beef, chicken and lamb fatty acid analysis - a simplified direct bimethylation procedure using freeze-dried material

M. R. F. Lee*, J. K. S. Tweed, E. J. Kim, N. D. Scollan

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

45 Citations (Scopus)

Abstract

When fractionation of meat lipids is not required. procedures such as saponification can be used to extract total fatty acids, reducing reliance on toxic organic compounds. However, saponification of muscle fatty acids is laborious, and requires extended heating times, and a second methylation step to convert the extracted fatty acids to fatty acid methyl esters prior to gas chromatography. Therefore the development of a more rapid direct methylation procedure would be of merit. The use of freeze-dried material for analysis is common and allows for greater homogenisation of the sample. The present study investigated the potential of using freeze-dried muscle samples and a direct bimethylation to analyse total fatty acids of meat (beef, chicken and lamb) in comparison with a saponification procedure followed by bimethylation. Both methods compared favourably for all major fatty acids measured. There was a minor difference in relation to the C18:1 trans 10 isomer with a greater (P

Original languageEnglish
Pages (from-to)863-866
Number of pages4
JournalMeat Science
Volume92
Issue number4
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Freeze-dried extraction
  • Saponification
  • Meat fatty acids
  • PURIFICATION
  • TISSUES
  • PHASE
  • SPECTROMETRY
  • Bimethylation
  • LIPID EXTRACTION
  • ISOMERS
  • CONJUGATED LINOLEIC-ACID
  • CHROMATOGRAPHY
  • TOXICITY
  • RAPID METHOD
  • Methylation

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