Bidirectional regulation of kainate receptor surface expression in hippocampal neurons

SG Martin, T Bouschet, EL Jenkins, A Nishimune, JM Henley

Research output: Contribution to journalArticle (Academic Journal)peer-review

28 Citations (Scopus)

Abstract

Kainate receptors (KARs) are crucial for the regulation of both excitatory and inhibitory neurotransmission but little is known regarding the mechanisms controlling KAR surface expression. We used Super Ecliptic pHluorin (SEP)-tagged KAR subunit GluR6a to investigate real time changes in KAR surface expression in hippocampal neurons. Sindbis virus expressed SEP-GluR6 subunits efficiently coassembled with native KAR subunits to form heteromeric receptors. Diffuse surface expressed dendritic SEP-GluR6 is rapidly internalized following either NMDA or kainate application. Sustained kainate or transient NMDA application resulted in a slow decrease of baseline surface KAR levels. Surprisingly, however, following the initial loss of surface receptors a short kainate application caused a long-lasting increase in surface expressed KARs to levels significantly greater than those prior to the agonist challenge. These data suggest that after initial endocytosis transient agonist activation evokes increased KAR exocytosis and reveal that KAR surface expression is bidirectionally regulated. This process may provide a mechanism for hippocampal neurons to differentially adapt their physiological responses to changes in synaptic activation and extrasynaptic glutamate concentration.
Translated title of the contributionBidirectional regulation of kainate receptor surface expression in hippocampal neurons
Original languageEnglish
Pages (from-to)36435 - 36440
Number of pages6
JournalJournal of Biological Chemistry
Volume283(52)
DOIs
Publication statusPublished - Dec 2008

Bibliographical note

Publisher: JBC

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