Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54

MF Fonseca; CJ Arthur; EHC Bromley; B Samyn; P Moerman; MJ Saavedra; A Correia; J Spencer

doi:10.1128/AAC.00429-11

Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54

MF Fonseca, CJ Arthur, EHC Bromley, B Samyn, P Moerman, MJ Saavedra, A Correia, J Spencer

Research output: Contribution to journal › Article (Academic Journal) › peer-review

14 Citations (Scopus)

Abstract

The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and over-expressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.

Translated title of the contribution	Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54
Original language	English
Pages (from-to)	5392 - 5395
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	55
DOIs	https://doi.org/10.1128/AAC.00429-11
Publication status	Published - Nov 2011

Access to Document

10.1128/AAC.00429-11

Handle.net

Persistent link

Cite this

@article{6da3451db4c54e0e86a6e3b94dbe7377,

title = "Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54",

abstract = "The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and over-expressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.",

author = "MF Fonseca and CJ Arthur and EHC Bromley and B Samyn and P Moerman and MJ Saavedra and A Correia and J Spencer",

year = "2011",

month = nov,

doi = "10.1128/AAC.00429-11",

language = "English",

volume = "55",

pages = "5392 -- 5395",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "1098-6596",

publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54

AU - Fonseca, MF

AU - Arthur, CJ

AU - Bromley, EHC

AU - Samyn, B

AU - Moerman, P

AU - Saavedra, MJ

AU - Correia, A

AU - Spencer, J

PY - 2011/11

Y1 - 2011/11

N2 - The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and over-expressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.

AB - The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and over-expressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.

U2 - 10.1128/AAC.00429-11

DO - 10.1128/AAC.00429-11

M3 - Article (Academic Journal)

C2 - 21876065

SN - 1098-6596

VL - 55

SP - 5392

EP - 5395

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

ER -

Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54

Abstract

Access to Document

Handle.net

Fingerprint

Cite this