Biochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54

MF Fonseca, CJ Arthur, EHC Bromley, B Samyn, P Moerman, MJ Saavedra, A Correia, J Spencer

Research output: Contribution to journalArticle (Academic Journal)peer-review

9 Citations (Scopus)

Abstract

The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and over-expressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.
Translated title of the contributionBiochemical characterization of Sfh-I, the subclass B2 metallo-{beta}-lactamase from Serratia fonticola UTAD54
Original languageEnglish
Pages (from-to)5392 - 5395
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume55
DOIs
Publication statusPublished - Nov 2011

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