Biochemical markers of the mechanical quality of engineered hyaline cartilage

DJ Kelly, A Crawford, SC Dickinson, TJ Sims, J Mundy, AP Hollander, PJ Prendergast, PV Hatton

Research output: Contribution to journalArticle (Academic Journal)peer-review

38 Citations (Scopus)

Abstract

The aim of this study was to determine whether or not biochemical markers can be used as surrogate measures for the mechanical quality of tissue engineered cartilage. The biochemical composition of tissue engineered cartilage constructs were altered by varying either (i) the initial cell seeding density of the scaffold (seeding density protocol) or (ii) the length of time the engineered tissue was cultured (culture period protocol). The aggregate or Young’s moduli of the constructs were measured (by confined or unconfined compression respectively), and compared with the composition of the extracellular matrix by quantitative measurement of the glycosaminoglycan (GAG), hydroxyproline, collagen I and collagen II and collagen cross-links. The aggregate modulus correlated positively with both GAG and collagen II content, but not with collagen I content. Young’s modulus correlated positively with GAG, collagen II and collagen I content, and the ratio of mature to immature cross-links. There was no significant correlation of Young’s Modulus with total collagen measured as hydroxyproline content. These results suggested that hydroxyproline determination may be an unreliable indicator of mechanical quality of tissue engineered cartilage, and that a measure of collagen II and GAG content is required to predict the biomechanical quality of tissue engineered cartilage.
Translated title of the contributionBiochemical markers of the mechanical quality of engineered hyaline cartilage
Original languageEnglish
Pages (from-to)273 - 281
Number of pages9
JournalJournal of Materials Science: Materials in Medicine
Volume18
DOIs
Publication statusPublished - Feb 2007

Bibliographical note

Publisher: Springer Netherlands

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