Biosynthesis and characterization of a novel, biocompatible medium chain length polyhydroxyalkanoate by Pseudomonas mendocina CH50 using coconut oil as the carbon source

Pooja Basnett, Elena Marcello, Barbara Lukasiewicz, Bijal Panchal, Rinat Nigmatullin, Jonathan Knowles, Ipsita Roy

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

This study validated the utilization of triacylglycerides (TAGs) by Pseudomonas mendocina CH50, a wild type strain, resulting in the production of novel mcl-PHAs with unique physical properties. A PHA yield of 58% dcw was obtained using 20 g/L of coconut oil. Chemical and structural characterisation confirmed that the mcl-PHA produced was a terpolymer comprising of three different repeating monomer units, 3-hydroxyoctanoate, 3-hydroxydecanoate and 3-hydroxydodecanoate or P(3HO-3HD-3HDD). Bearing in mind the potential of P(3HO-3HD-3HDD) in biomedical research, especially in neural tissue engineering, in vitro biocompatibility studies were carried out using NG108-15 (neuronal) cells. Cell viability data confirmed that P(3HO-3HD-3HDD) supported the attachment and proliferation of NG108-15 and was therefore confirmed to be biocompatible in nature and suitable for neural regeneration.
Original languageEnglish
Article number179
Number of pages11
JournalJournal of Materials Science: Materials in Medicine
Volume29
Early online date30 Nov 2018
DOIs
Publication statusPublished - 30 Nov 2018

Fingerprint

Dive into the research topics of 'Biosynthesis and characterization of a novel, biocompatible medium chain length polyhydroxyalkanoate by <i>Pseudomonas mendocina</i> CH50 using coconut oil as the carbon source'. Together they form a unique fingerprint.

Cite this