Projects per year
Abstract
Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.
Original language | English |
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Pages (from-to) | 5501-5507 |
Number of pages | 7 |
Journal | Journal of the American Chemical Society |
Volume | 136 |
Issue number | 14 |
DOIs | |
Publication status | Published - 9 Apr 2014 |
Research Groups and Themes
- Bristol BioDesign Institute
Keywords
- synthetic biology
- ACYL CARRIER PROTEINS
- REVEALS
- GENE-CLUSTER
- MUTATIONAL ANALYSIS
- ACID-B
- ANTIBIOTIC MUPIROCIN
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Dive into the research topics of 'Biosynthesis of Mupirocin by Pseudomonas fluorescens NCIMB 10586 Involves Parallel Pathways'. Together they form a unique fingerprint.Projects
- 3 Finished
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Revised: Engineering Trans-AT PKS antibiotic gene cluster to deliver bioactive compounds
Willis, C. L. (Principal Investigator)
31/07/14 → 31/03/22
Project: Research
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3-month Core Capability for Chemistry Research
Crosby, J. (Principal Investigator)
1/01/13 → 1/04/13
Project: Research
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CHEMICAL ANALYSIS OF HYBRID FUNGAL MEGASYNTHASES
Cox, R. J. (Principal Investigator)
1/10/08 → 1/10/12
Project: Research
Profiles
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Professor Matthew P Crump
- School of Chemistry - Professor of NMR and Structural Biology
- Cancer
Person: Academic , Member