Biosynthesis of Mupirocin by Pseudomonas fluorescens NCIMB 10586 Involves Parallel Pathways

Shu-Shan Gao, Joanne Hothersall, Ji'en Wu, Annabel C. Murphy, Zhongshu Song, Elton R. Stephens, Christopher M. Thomas, Matthew P. Crump, Russell J. Cox, Thomas J. Simpson*, Christine L. Willis

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

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Abstract

Mupirocin, a clinically important antibiotic produced via a trans-AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens, consists of a mixture of mainly pseudomonic acids A, B, and C. Detailed metabolic profiling of mutant strains produced by systematic inactivation of PKS and tailoring genes, along with re-feeding of isolated metabolites to mutant stains, has allowed the isolation of a large number of novel metabolites, identification of the 10,11-epoxidase, and full characterization of the mupirocin biosynthetic pathway, which proceeds via major (10,11-epoxide) and minor (10,11-alkene) parallel pathways.

Original languageEnglish
Pages (from-to)5501-5507
Number of pages7
JournalJournal of the American Chemical Society
Volume136
Issue number14
DOIs
Publication statusPublished - 9 Apr 2014

Keywords

  • ACYL CARRIER PROTEINS
  • ACID-B
  • GENE-CLUSTER
  • ANTIBIOTIC MUPIROCIN
  • MUTATIONAL ANALYSIS
  • REVEALS

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