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Biosynthesis of thiomarinol A and related metabolites of Pseudoalteromonas sp SANK 73390

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalChemical Science
Volume5
Issue number1
Early online date1 Nov 2013
DOIs
DateAccepted/In press - 31 Oct 2013
DateE-pub ahead of print - 1 Nov 2013
DatePublished (current) - 1 Jan 2014

Abstract

The biosynthesis of the mixed PKS-FAS-NRPS hybrid antibiotic thiomarinol A was investigated using feeding studies to both wild type and mutant strains of the marine bacterium Pseudoalteromonas. Particularly interesting features of the pathway include assembly of the 8-hydroxyoctanoic acid side-chain via chain extension of a C4-precursor (4-hydroxybutyrate), and construction of the pyrrothine unit from cysteine via (HolA-D, F-H) prior to intact incorporation into thiomarinol (catalysed by TmlU). A series of thiomarinol-related and other minor metabolites have been isolated from wild-type and mutant strains. The results of these investigations are rationalised in terms of the overall biosynthetic pathway.

    Research areas

  • PSEUDOMONIC-ACID-A, MARINE BACTERIUM, ANTIMICROBIAL ACTIVITY, GENE-CLUSTER, FLUORESCENS, ANTIBIOTICS, MUPIROCIN, XENORHABDUS, HOLOMYCIN, PATHWAY

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Royal Society of Chemistry at http://dx.doi.org/10.1039/C3SC52281D. Please refer to any applicable terms of use of the publisher.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Royal Society of Chemistry at http://dx.doi.org/10.1039/C3SC52281D. Please refer to any applicable terms of use of the publisher.

    Final published version, 1 MB, PDF document

    Licence: CC BY

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