Biosynthesis of very long chain polyunsaturated fatty acids in the leafy vegetable chicory

H Mekky, M Mohamed, C Lazarus, JB Power, MR Davey

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)

Abstract

The synthesis of very long chain polyunsaturated fatty acids (VLCPUFAs) was investigated in five cultivars of chicory. Genes for enzymes of the ω3/6 Δ8-desaturation biosynthetic pathways for the formation of C20 VLCPUFAs were inserted into chicory by Agrobacterium-mediated transformation of leaf explants. Plants were transformed by genes encoding Δ9-specific elongating activity from Isochrysis galbana, Δ8-desaturase from Euglena gracilis and Δ5-desaturase from Mortierella alpina, either separately or in combination; transgenic plants were selected on culture medium containing glufosinate ammonium for those transformed with the Δ9-elongase gene alone or in combination with the Δ8-desaturase gene, or kanamycin for plants transformed with the Δ5-desaturase gene. PCR showed the presence of the transgenes within the genome of selected plants, with RT-PCR confirming gene expression. Gas Chromatography of fatty acid methyl esters extracted from freeze-dried leaves of transgenic plants quantified the synthesis of omega-6 arachidonic acid and its precursors eicosadienoic and dihomo-γ-linolenic acids, and omega-3 eicosapentaenoic acid together with its precursors eicosatrienoic and eicosatetraenoic acids. This is the first report of the production of VLCPUFAs in a leafy vegetable. Since VLCPUFAs are the precursors of prostaglandins, the formation of prostaglandins was also investigated in chicory following a second transformation event using the PGHS-1 gene from Mus musculus.
Translated title of the contributionBiosynthesis of very long chain polyunsaturated fatty acids in the leafy vegetable chicory
Original languageEnglish
Pages (from-to)327 - 340
Number of pages14
JournalAgricultural and Food Science
Volume20
DOIs
Publication statusPublished - Oct 2011

Fingerprint Dive into the research topics of 'Biosynthesis of very long chain polyunsaturated fatty acids in the leafy vegetable chicory'. Together they form a unique fingerprint.

Cite this