Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells

Sarah Glennie, Inês Soeiro, Peter J. Dyson, Eric W.F. Lam, Francesco Dazzi*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

870 Citations (Scopus)

Abstract

It has been shown that mesenchymal stem cells (MSCs) induce T cells to become unresponsive. We characterized the phenotype of these T cells by dissecting the effect of MSCs on T-cell activation, proliferation, and effector function. For this purpose, an in vitro murine model was used in which T-cell responses were generated against the male HY minor histocompatibility antigen. In the presence of MSCs, the expression of early activation markers CD25 and CD69 was unaffected but interferon-γ (IFN-γ) production was reduced. The inhibitory effect of MSCs was directed mainly at the level of cell proliferation. Analysis of the cell cycle showed that T cells, stimulated in the presence of MSCs, were arrested at the G1 phase. At the molecular level, cyclin D2 expression was profoundly inhibited, whereas p27kip1 was up-regulated. When MSCs were removed from the cultures and restimulated with the cognate peptide, T cells produced IFN-γ but failed to proliferate. The addition of exogenous interleukin-2 (IL-2) did not restore proliferation. MSCs did not preferentially target any T-cell subset, and the inhibition was also extended to B cells. MSC-mediated inhibition induces an unresponsive T-cell profile that is fully consistent with that observed in division arrest anergy.

Original languageEnglish
Pages (from-to)2821-2827
Number of pages7
JournalBlood
Volume105
Issue number7
DOIs
Publication statusPublished - 1 Apr 2005

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    Glennie, S., Soeiro, I., Dyson, P. J., Lam, E. W. F., & Dazzi, F. (2005). Bone marrow mesenchymal stem cells induce division arrest anergy of activated T cells. Blood, 105(7), 2821-2827. https://doi.org/10.1182/blood-2004-09-3696