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Bone marrow transplantation stimulates neural repair in Friedreich's ataxia mice

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Bone marrow transplantation stimulates neural repair in Friedreich's ataxia mice. / Kemp, Kevin; Hares, Kelly; Redondo, Juliana; Cook, Amelia; Haynes, Harry; Burton, Bronwen; Pook, Mark ; Rice, Claire; Scolding, Neil; Wilkins, Alastair.

In: Annals of Neurology, Vol. 83, No. 4, 23.04.2018, p. 779-793.

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@article{0ef713df52004e35a89371be8d5240c8,
title = "Bone marrow transplantation stimulates neural repair in Friedreich's ataxia mice",
abstract = "OBJECTIVES:Friedreich's ataxia is an incurable inherited neurological disease caused by frataxin deficiency. Here we report the neuro-reparative effects of myeloablative allogeneic bone marrow transplantation in a humanised murine model of the disease.METHODS:Mice received a transplant of fluorescently-tagged sex mis-matched bone marrow cells expressing wild-type frataxin and were assessed at monthly intervals using a range of behavioural motor performance tests. At six months post-transplant, mice were sacrificed for protein and histological analysis. In an attempt to augment numbers of bone marrow-derived cells integrating within the nervous system and improve therapeutic efficacy, a sub-group of transplanted mice also received monthly subcutaneous infusions of cytokines granulocyte-colony stimulating factor and stem cell factor.RESULTS:Transplantation caused improvements in several indicators of motor coordination and locomotor activity. Elevations in frataxin levels and anti-oxidant defences were detected. Abrogation of disease pathology throughout the nervous system was apparent, together with extensive integration of bone marrow-derived cells in areas of nervous tissue injury that contributed genetic material to mature neurons, satellite-like cells and myelinating Schwann cells by processes including cell fusion. Elevations in circulating bone marrow-derived cell numbers were detected post-cytokine administration and were associated with increased frequencies of Purkinje cell fusion and bone marrow-derived dorsal root ganglion satellite-like cells. Further improvements in motor coordination and activity were evident.INTERPRETATION:Our data provide proof-of-concept of gene replacement therapy, via allogeneic bone marrow transplantation, that reverses neurological features of Friedreich's ataxia with the potential for rapid clinical translation. This article is protected by copyright. All rights reserved.",
author = "Kevin Kemp and Kelly Hares and Juliana Redondo and Amelia Cook and Harry Haynes and Bronwen Burton and Mark Pook and Claire Rice and Neil Scolding and Alastair Wilkins",
year = "2018",
month = "4",
day = "23",
doi = "10.1002/ana.25207",
language = "English",
volume = "83",
pages = "779--793",
journal = "Annals of Neurology",
issn = "0364-5134",
publisher = "Wiley",
number = "4",

}

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TY - JOUR

T1 - Bone marrow transplantation stimulates neural repair in Friedreich's ataxia mice

AU - Kemp, Kevin

AU - Hares, Kelly

AU - Redondo, Juliana

AU - Cook, Amelia

AU - Haynes, Harry

AU - Burton, Bronwen

AU - Pook, Mark

AU - Rice, Claire

AU - Scolding, Neil

AU - Wilkins, Alastair

PY - 2018/4/23

Y1 - 2018/4/23

N2 - OBJECTIVES:Friedreich's ataxia is an incurable inherited neurological disease caused by frataxin deficiency. Here we report the neuro-reparative effects of myeloablative allogeneic bone marrow transplantation in a humanised murine model of the disease.METHODS:Mice received a transplant of fluorescently-tagged sex mis-matched bone marrow cells expressing wild-type frataxin and were assessed at monthly intervals using a range of behavioural motor performance tests. At six months post-transplant, mice were sacrificed for protein and histological analysis. In an attempt to augment numbers of bone marrow-derived cells integrating within the nervous system and improve therapeutic efficacy, a sub-group of transplanted mice also received monthly subcutaneous infusions of cytokines granulocyte-colony stimulating factor and stem cell factor.RESULTS:Transplantation caused improvements in several indicators of motor coordination and locomotor activity. Elevations in frataxin levels and anti-oxidant defences were detected. Abrogation of disease pathology throughout the nervous system was apparent, together with extensive integration of bone marrow-derived cells in areas of nervous tissue injury that contributed genetic material to mature neurons, satellite-like cells and myelinating Schwann cells by processes including cell fusion. Elevations in circulating bone marrow-derived cell numbers were detected post-cytokine administration and were associated with increased frequencies of Purkinje cell fusion and bone marrow-derived dorsal root ganglion satellite-like cells. Further improvements in motor coordination and activity were evident.INTERPRETATION:Our data provide proof-of-concept of gene replacement therapy, via allogeneic bone marrow transplantation, that reverses neurological features of Friedreich's ataxia with the potential for rapid clinical translation. This article is protected by copyright. All rights reserved.

AB - OBJECTIVES:Friedreich's ataxia is an incurable inherited neurological disease caused by frataxin deficiency. Here we report the neuro-reparative effects of myeloablative allogeneic bone marrow transplantation in a humanised murine model of the disease.METHODS:Mice received a transplant of fluorescently-tagged sex mis-matched bone marrow cells expressing wild-type frataxin and were assessed at monthly intervals using a range of behavioural motor performance tests. At six months post-transplant, mice were sacrificed for protein and histological analysis. In an attempt to augment numbers of bone marrow-derived cells integrating within the nervous system and improve therapeutic efficacy, a sub-group of transplanted mice also received monthly subcutaneous infusions of cytokines granulocyte-colony stimulating factor and stem cell factor.RESULTS:Transplantation caused improvements in several indicators of motor coordination and locomotor activity. Elevations in frataxin levels and anti-oxidant defences were detected. Abrogation of disease pathology throughout the nervous system was apparent, together with extensive integration of bone marrow-derived cells in areas of nervous tissue injury that contributed genetic material to mature neurons, satellite-like cells and myelinating Schwann cells by processes including cell fusion. Elevations in circulating bone marrow-derived cell numbers were detected post-cytokine administration and were associated with increased frequencies of Purkinje cell fusion and bone marrow-derived dorsal root ganglion satellite-like cells. Further improvements in motor coordination and activity were evident.INTERPRETATION:Our data provide proof-of-concept of gene replacement therapy, via allogeneic bone marrow transplantation, that reverses neurological features of Friedreich's ataxia with the potential for rapid clinical translation. This article is protected by copyright. All rights reserved.

UR - http://www.scopus.com/inward/record.url?scp=85045769523&partnerID=8YFLogxK

U2 - 10.1002/ana.25207

DO - 10.1002/ana.25207

M3 - Article

C2 - 29534309

VL - 83

SP - 779

EP - 793

JO - Annals of Neurology

JF - Annals of Neurology

SN - 0364-5134

IS - 4

ER -