Projects per year
High-speed atomic force microscopy (AFM) is important for following processes that occur on sub-second timescales for studies both in biology and materials science, and also for the ability to examine large areas of a specimen at high resolution in a practical length of time. Further developments of the previously reported high-speed contact-mode AFM are described. Two instruments are presented: (i) a high-speed flexure stage arrangement capable of imaging at a video rate of 30 fps, and (ii) an ultra-high speed instrument using a combined tuning fork and flexure-stage scanning system capable of ultra-high-speed imaging in excess of 1000 fps. Results of imaging collagen fibres under ambient conditions at rates of up to 1300 frames s-1 are presented. Despite tipâ€“specimen relative velocities of up to 200 mm s-1, no significant damage to the collagen specimen was observed even after tens of thousands of frames were acquired in the same area of the specimen.
Bibliographical notePublisher: Institute of Physics Publishing
Picco, LM., Bozec, L., Ulcinas, A., Engledew, DJ., Antognozzi, M., Horton, MA., & Miles, MJ. (2007). Breaking the speed limit with atomic force microscopy. Nanotechnology, 18(4), 044030/1 - 044030/4. https://doi.org/10.1088/0957-4484/18/4/044030