Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2

H Mellor, S R Kimball, L S Jefferson

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).

Original languageEnglish
Pages (from-to)143-6
Number of pages4
JournalFEBS Letters
Volume350
Issue number1
DOIs
Publication statusPublished - 15 Aug 1994

Keywords

  • Animals
  • Blotting, Western
  • Brefeldin A
  • Cyclopentanes/pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Eukaryotic Initiation Factor-2/chemistry
  • Peptide Chain Initiation, Translational/drug effects
  • Phosphorylation
  • Protein Synthesis Inhibitors/pharmacology
  • Rats
  • Tumor Cells, Cultured

Fingerprint Dive into the research topics of 'Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2'. Together they form a unique fingerprint.

Cite this