Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2

H Mellor; S R Kimball; L S Jefferson

doi:10.1016/0014-5793(94)00756-x

Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2

H Mellor, S R Kimball, L S Jefferson

Research output: Contribution to journal › Article (Academic Journal) › peer-review

15 Citations (Scopus)

Abstract

Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).

Original language	English
Pages (from-to)	143-6
Number of pages	4
Journal	FEBS Letters
Volume	350
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)00756-x
Publication status	Published - 15 Aug 1994

Keywords

Animals
Blotting, Western
Brefeldin A
Cyclopentanes/pharmacology
Electrophoresis, Polyacrylamide Gel
Eukaryotic Initiation Factor-2/chemistry
Peptide Chain Initiation, Translational/drug effects
Phosphorylation
Protein Synthesis Inhibitors/pharmacology
Rats
Tumor Cells, Cultured

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title = "Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2",

abstract = "Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).",

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author = "H Mellor and Kimball, {S R} and Jefferson, {L S}",

year = "1994",

month = aug,

day = "15",

doi = "10.1016/0014-5793(94)00756-x",

language = "English",

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journal = "FEBS Letters",

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T1 - Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2

AU - Mellor, H

AU - Kimball, S R

AU - Jefferson, L S

PY - 1994/8/15

Y1 - 1994/8/15

N2 - Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).

AB - Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).

KW - Animals

KW - Blotting, Western

KW - Brefeldin A

KW - Cyclopentanes/pharmacology

KW - Electrophoresis, Polyacrylamide Gel

KW - Eukaryotic Initiation Factor-2/chemistry

KW - Peptide Chain Initiation, Translational/drug effects

KW - Phosphorylation

KW - Protein Synthesis Inhibitors/pharmacology

KW - Rats

KW - Tumor Cells, Cultured

U2 - 10.1016/0014-5793(94)00756-x

DO - 10.1016/0014-5793(94)00756-x

M3 - Article (Academic Journal)

C2 - 8062914

SN - 0014-5793

VL - 350

SP - 143

EP - 146

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Brefeldin A inhibits protein synthesis through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2

Abstract

Keywords

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