Abstract
Brefeldin A is a fungal metabolite which disrupts protein traffic through the Golgi apparatus and thereby inhibits protein secretion. Recently, it has been shown that Brefeldin A also causes a marked decrease in the rate of protein synthesis in cells in culture [1992, FEBS Lett. 314, 371-374]. We show here that treatment of rat GH3 pituitary cells with Brefeldin A leads to an inhibition of protein synthesis at the level of peptide-chain initiation through a mechanism involving the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF-2 alpha).
| Original language | English |
|---|---|
| Pages (from-to) | 143-6 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 350 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 15 Aug 1994 |
Keywords
- Animals
- Blotting, Western
- Brefeldin A
- Cyclopentanes/pharmacology
- Electrophoresis, Polyacrylamide Gel
- Eukaryotic Initiation Factor-2/chemistry
- Peptide Chain Initiation, Translational/drug effects
- Phosphorylation
- Protein Synthesis Inhibitors/pharmacology
- Rats
- Tumor Cells, Cultured
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