Bromocriptine reduces rat thyrotropin beta-subunit mRNA stability

A Levy, S L Lightman

Research output: Contribution to journalArticle (Academic Journal)peer-review

3 Citations (Scopus)

Abstract

We have examined the effect of orally administered bromocriptine on TSH beta-subunit messenger (m)RNA in the anterior pituitary glands of Sprague-Dawley rats using in situ and dot-blot hybridization histochemistry. Quantitative in situ hybridization of pituitary sections demonstrated a 60% reduction in TSH beta-subunit mRNA probe binding from rats fed a diet containing bromocriptine 10 mg/kg/day. This was confirmed by dot-blot analysis of nuclear and cytoplasmic pituitary extracts from the same tissue. Hybridization of cytoplasmic extracts of pituitary cells cultured under actinomycin D-induced transcription arrest showed that part of the effect of bromocriptine appeared to be mediated through a change in TSH beta-subunit mRNA stability and implies that the acute influence of dopamine on TSH metabolism may be transduced by control of TSH beta-subunit mRNA catabolism. This suggests a mechanism by which cells with relatively stable tissue specific mRNAs appear to respond rapidly to hormonal effects at the transcriptional level.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalJournal of Endocrinological Investigation
Volume13
Issue number1
Publication statusPublished - Jan 1990

Keywords

  • Administration, Oral
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Bromocriptine
  • Cells, Cultured
  • Dactinomycin
  • Dopamine
  • Gene Expression Regulation
  • Male
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Pituitary Gland, Anterior
  • RNA, Messenger
  • Rats
  • Rats, Inbred Strains
  • Thyrotropin
  • Transcription, Genetic

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