Calcium Binding in α-Amylases: An X-ray Diffraction Study at 2.1-Å Resolution of Two Enzymes from Aspergillus

E. Boel, L. Brady, A. M. Brzozowski, Z. Derewenda*, G. G. Dodson, V. J. Jensen, S. B. Petersen, H. Swift, L. Thim, H. F. Woldike

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

305 Citations (Scopus)

Abstract

X-ray diffraction analysis (at 2.1-Å resolution) of an acid α-amylase from Aspergillus niger allowed a detailed description of the stereochemistry of the calcium-binding sites. The primary site (which is essential in maintaining proper folding around the active site) contains a tightly bound Ca2+ with an unusually high number of eight ligands (051 and 052 of Aspl75, 05 of Asnl21, main-chain carbonyl oxygens of Glu 162 and Glu210, and three water molecules). A secondary binding site was identified at the bottom of the substrate binding cleft; it involves the residues presumed to play a catalytic role (Asp206 and Glu230). This explains the inhibitory effect of calcium observed at higher concentrations. Neutral Aspergillus oryzae (TAKA) α-amylase was also refined in a new crystal at 2.1-Å resolution. The structure of this homologous (over 80%) enzyme and additional kinetic studies support all the structural conclusions regarding both calcium-binding sites.

Original languageEnglish
Pages (from-to)6244-6249
Number of pages6
JournalBiochemistry
Volume29
Issue number26
DOIs
Publication statusPublished - 1 Jul 1990

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